盐地碱蓬内生菌Neocamarosporium sp.ZLM-26次级代谢产物  

Secondary metabolites of an endophytic fungus Neocamarosporium sp.ZLM-26 from Suaeda salsa

作　　者：张迪 郑金扬 曾丽媚 张全 冯瑶 司鑫鑫 石晓 ZHANG Di;ZHENG Jinyang;ZENG Limei;ZHANG Quan;FENG Yao;SI Xinxin;SHI Xiao(Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,School of Pharmacy,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China)

机构地区：[1]江苏海洋大学药学院,江苏省海洋药物活性分子筛选重点实验室,江苏连云港222005

出　　处：《菌物学报》2023年第6期1396-1403,共8页Mycosystema

基　　金：江苏海洋大学人才引进项目(KQ20047);江苏省海洋药物活性分子筛选重点实验室开放课题(HY202001);江苏省“双创”博士(JSSCBS20211307)~~。

摘　　要：本研究对盐地碱蓬内生菌Neocamarosporium sp.ZLM-26的活性次级代谢产物进行了挖掘。采用薄层色谱、硅胶柱色谱、高效液相色谱等分离方法,从该菌株大米培养基发酵产物的乙酸乙酯萃取物中分离得到6个单体化合物,经波谱学解析和文献数据对比,鉴定6个化合物分别为:5-butyl-6-(hydroxymethyl)-2H-pyran-2-one(1)、(7S)-xylariolide E(2)、(6S)-xylariolide D(3)、diaporpyrone A(4)、4-hydroxybenzaldehyde(5)和2-(2-hydroxyethyl)phenol(6)。分别采用CCK-8法和96孔板法评估了6个化合物的抗肿瘤和抗菌活性。结果显示,6个化合物(50μmol/L)对胰腺癌细胞株SW1990、PANC-1的增殖无明显抑制作用;而化合物4对大肠杆菌有较好的增殖抑制活性,化合物5、6对铜绿假单胞菌有较好的增殖抑制活性。化合物1为1个新的天然产物,本研究首次报道了其核磁数据和抗菌活性。此外,化合物1–4均为首次从新凸轮孢菌属真菌中分离得到。The bioactive secondary metabolites of Suaeda salsa endophyte Neocamarosporium sp.ZLM-26 was explored.Using the methods of thin layer chromatography,column chromatography of silica gel and high performance liquid chromatography,six compounds were isolated from the ethyl acetate extract of ZLM-26's rice medium.Their structures were elucidated by spectral analysis and the literature data comparison,and they were identified as 5-butyl-6-(hydroxymethyl)-2H-pyran-2-one(1),(7S)-xylariolide E(2),(6S)-xylariolide D(3),diaporpyrone A(4),4-hydroxybenzaldehyde(5)and 2-(2-hydroxyethyl)phenol(6).The antitumor and antimicrobial activities of these compounds were evaluated by CCK-8 and 96-well plate methods,respectively.Six compounds showed no antitumor activities on either HGC-27 or PANC-1 cell lines at the concentration of 50μmol/L.Compound 4 showed relatively efficient anti-proliferation activities against Escherichia coli,while compound 5 and 6 had a moderate activity of inhibition of Pseudomonas aeruginosa.The NMR data and antibacterial activity of compound 1,a new natural product,were reported for the first time.Additionally,compounds 1–4were isolated from Neocamarosporium for the first time.

关 键 词：盐地碱蓬内生菌 Neocamarosporium sp.ZLM-26 次级代谢产物 抗肿瘤活性 抗菌活性 

分 类 号：Q949.9[生物学—植物学]