常压低温等离子体对羊肚菌培养基质的杀菌效果及工艺优化  

Sterilization effects on morel culture substrate of technologically optimized low temperature plasma

作　　者：王秀茹 沈振兴[1] 杨一鸣 樊灏 杨柳[1] 孙健 王涛[2] 李方向 WANG Xiuru;SHEN Zhenxing;YANG Yiming;FAN Hao;YANG Liu;SUN Jian;WANG Tao;LI Fangxiang(School of Energy and Power Engineering,Xi'an Jiaotong University,Xi'an 710049,Shaanxi,China;Xi'an Agricultural Technology Extension Center,Xi'an 710061,Shaanxi,China)

机构地区：[1]西安交通大学能源与动力工程学院,陕西西安710049 [2]西安市农业技术推广中心,陕西西安710061

出　　处：《菌物学报》2023年第6期1413-1422,共10页Mycosystema

基　　金：西安市科学技术局农业技术研发项目(21NYYF0041)。

摘　　要：以羊肚菌培养基质为试验对象,采用常压低温等离子体技术对其进行杀菌处理,分别研究了等离子体处理过程中电压、频率、时间和次数4种单因素变量对菌落数和水分的影响,并通过正交实验优化了处理工艺条件。结果表明,细菌和真菌的杀菌率随常压等离子体处理电压、处理时间和处理次数的增大而增加,水分损失也随之增加,而随着频率的增大,杀菌率呈现先增大后减小的“火山型”趋势。不同处理条件下的杀菌效果普遍存在显著性差异(P<0.05)。正交实验表明4种影响因素大小为:频率>电压>时间>次数,最佳杀菌工艺条件为:电压30kV,频率9.6kHz,时间45 min,次数1次,此条件下细菌和真菌杀菌率分别为96.87%和93.77%,且水分损失较少。Morel culture substrate was sterilized by plasma technology at normal pressure and low temperature.The effects of four single factor variables,voltage,frequency,treatment time and treatment times,on colony number and water content were investigated,and the treatment conditions were optimized by orthogonal test.The results showed that the sterilization rate of bacteria and fungi increased with the increase of voltage,time and times of atmospheric plasma treatment,and the water loss also increased.With the increase of plasma treatment frequency,the sterilization rate first increased and then decreased,showing a tendency of“volcanic type”.There were significant differences in germicidal effect under different treatment conditions(P0.05).The orthogonal test showed that the influencing degree of the four factors was ranged as frequencyvoltagetreatment timetreatment times.The optimal sterilization condition was as follows:voltage 30 kV,frequency 9.6 kHz,time 45 min,once a treatment.Under this condition,the sterilization rates of bacteria and fungi were 96.87%and 93.77%,respectively,and the water loss was less.

关 键 词：低温等离子体 羊肚菌培养料 杀菌率 正交实验 

分 类 号：S646.7[农业科学—蔬菜学]