一株白囊耙齿菌对刚果红染料的脱色研究及其毒力测试  

Decolorization and detoxification of Congo red by Irpex lacteus strain 2-1-1

作　　者：颜倩 李国芝 尚晓静 侯瑞[1] YAN Qian;LI Guozhi;SHANG Xiaojing;HOU Rui(College of Forestry,Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州大学林学院,贵州贵阳550025

出　　处：《菌物学报》2023年第6期1423-1434,共12页Mycosystema

基　　金：国家自然科学基金(32260651);贵州省科技计划(黔科合支撑[2017]2567,黔科合平台人才[2018]5781)。

摘　　要：菌株2-1-1于贵州大学校园樱花树干子实体中分离,通过形态学结构、ITS序列及系统发育树分析,鉴定其为白囊耙齿菌Irpex lacteus,对菌株2-1-1产生的3种木质素降解酶进行测定,发现菌株可分泌漆酶(laccase,Lac)、木质素过氧化物酶(lignin peroxidase,LiP)和锰过氧化物酶(manganese peroxidase,MnP)。利用菌株2-1-1对固体培养基条件下7种染料的脱色能力进行检测,筛选出较易脱色的刚果红染料,同时采用气相色谱-质谱联用(gas chromatography-mass spectroscopy,GC-MS)分析确定经白囊耙齿菌降解的刚果红染料代谢产物,并对其进行脱色条件优化和毒力测验,试验分析表明:菌株2-1-1对7种染料均有脱色,对刚果红脱色效果较佳。GC-MS分析刚果红染料降解产物主要为联苯胺、联苯、苯乙烯、十六烷和3-硝基苯酚。单因素和综合优化脱色条件:染料浓度50mg/L,碳源为果糖、金属离子为锌离子、pH7,该条件下刚果红染料脱色效果最优。优化条件下菌株2-1-1对刚果红染料脱色率达91.03%,相比对照组脱色率提高17.13%,刚果红染料经菌株2-1-1脱色前后毒力测试:染料原液>脱色后>清水处理,表明该菌株可降低刚果红染料毒性。Irpex lacteus strain 2-1-1 was isolated from wild fruiting body on cherry tree trunk in campus of Guizhou University and identified on the basis of morphological structure,ITS sequencing,and phylogenetic analysis.Its three lignin-degrading enzymes were identified as laccase(Lac),lignin peroxidase(LiP),and manganese peroxidase(MnP).Decolorization experiment on seven dyes in solid medium proved that I.lacteus 2-1-1 could decolorize all the seven dyes,and Congo red dye was most easily decolorized.According to gas chromatography-mass spectrometry(GC-MS),the main degradation products of Congo red dyes were benzidine,biphenyl,styrene,hexadecane,and 3-nitrophenol.Single factor and comprehensive optimization of decolorization conditions are that under Congo red dye concentration of 50 mg/L,the best carbon source is fructose,metal ion is Zn2+,and pH is 7.Under these conditions,the decolorization rate of Congo red was 91.03%,and increased by 17.13%as compared with the experimental control.The toxicity test of Congo red dye before and after decolorization by I.lacteus indicated that after clear water treatment,the toxicity of Congo red dye was obviously reduced.

关 键 词：白囊耙齿菌 木质素降解酶 刚果红 脱色优化 毒性 

分 类 号：X703[环境科学与工程—环境工程] X172