毛蕊异黄酮通过调节线粒体通路抑制胃癌AGS细胞的增殖、迁移和侵袭  

Calycosin Inhibits the Proliferation,Migration and Invasion of Gastric Cancer AGS Cells by Modulating the Mitochondrial Pathway

作　　者：李倩慧 平仙娥 LI Qian-Hui;PING Xian-E(Jiading District Anting Hospital,Shanghai 201805,China)

机构地区：[1]上海市嘉定区安亭医院,上海201805

出　　处：《广州中医药大学学报》2023年第6期1482-1487,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：上海市卫生系统优秀青年医学人才培养项目(编号:PWRq2018-11)。

摘　　要：【目的】探讨毛蕊异黄酮对人胃癌AGS细胞的增殖、迁移和侵袭机制。【方法】培养人胃癌细胞系AGS,使用毛蕊异黄酮或/和MHY1485[哺乳动物雷帕霉素靶蛋白(mTOR)激活剂]处理胃癌细胞,采用四甲基偶氮唑盐(MTT)法检测细胞增殖能力,Transwell法检测细胞迁移、侵袭能力,JC-1免疫荧光染色法检测线粒体膜电位变化,Western Blot法检测自噬关键蛋白微管相关蛋白1轻链3(LC3)-Ⅰ、LC3-Ⅱ、Beclin-1、磷酸化mTOR(p-mTOR)蛋白表达。【结果】毛蕊异黄酮抑制AGS细胞的增殖与迁移、侵袭能力,上调线粒体膜电位,促进细胞自噬发生,下调Beclin-1与LC3-Ⅱ的表达,抑制p-mTOR的表达。MHY1485共处理部分逆转毛蕊异黄酮对AGS细胞增殖、迁移、侵袭的抑制。【结论】毛蕊异黄酮可通过抑制p-mTOR表达,促进AGS细胞自噬发生,进而抑制AGS细胞的增殖、迁移与侵袭。Objective To investigate the proliferation,migration and invasion mechanism of calycosin on human gastric cancer AGS cells.Methods The human gastric cancer cell line AGS was cultured,and the gastric cancer ASC cells were treated with calycosin or/and MHY1485[mammalian target of rapamycin(mTOR)activator].The proliferation ability of the cells were detected by tetramethylazole salt(MTT)method,the migration and invasion abilities of the cells were detected by Transwell method,and the mitochondrial membrane potential changes were detected by JC-1 immunofluorescence staining.Western Blot was used to detect the protein expressions of microtubule-associated protein 1 light chain 3(LC3)-Ⅰ,LC3-Ⅱ,Beclin-1 and phosphorylated mTOR(p-mTOR),which were key proteins for autophagy.Results Calycosin inhibited the proliferation,migration and invasion abilities of AGS cells,up-regulated the mitochondrial membrane potential of cells,promoted autophagy,down-regulated the expressions of Beclin-1 and LC3-Ⅱ,and inhibited the expression of p-mTOR.MHY1485 co-treatment partially reversed the inhibition of calycosin on proliferation,migration and invasion abilities of AGS cells.Conclusion Calycosin promoted autophagy in AGS cells by inhibiting the expression of p-mTOR,which in turn inhibited the proliferation,migration and invasion of AGS cells.

关 键 词：毛蕊异黄酮 胃癌 细胞增殖 细胞迁移 细胞侵袭 线粒体膜电位 AGS细胞系 

分 类 号：R285.5[医药卫生—中药学]