消渴丸对2型糖尿病db/db小鼠降压降脂的拆方研究  

Study on the Regularity of Recipe Composition of Xiaoke Pills forHypotensive and Hypolipidemic Effects in Type 2 Diabetic db/db Mice

作　　者：党蕾 宁娜 黄秋凌 陈健钊 刘珊珊 DANG Lei;NING Na;HUANG Qiu-Ling;CHEN Jian-Zhao;LIU Shan-Shan(Guangzhou Baiyunshan Zhongyi Pharmaceutical Co.Ltd.,Guangzhou 510530 Guangdong,China;Guangzhou General Pharmaceutical Research Institute Co.,Ltd.,Guangzhou 510240 Guangdong,China)

机构地区：[1]广州白云山中一药业有限公司,广东广州510530 [2]广州医药研究总院有限公司,广东广州510240

出　　处：《广州中医药大学学报》2023年第5期1228-1234,共7页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】探讨消渴丸对2型糖尿病db/db小鼠的降压降脂作用。【方法】通过拆方方法,比较消渴丸低剂量组(1.0 g/kg)、消渴丸高剂量组(4.0 g/kg)、消渴丸纯中药组(3.996 g/kg)和格列本脲组(4.0 mg/kg)自发性糖尿病db/db小鼠给药前后收缩压和舒张压、空腹血糖、胰岛素、胰岛素抵抗指数(HOMA-IR)、血清总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)的变化。比较给药处理后,各组间血清血管紧张素Ⅱ(AngⅡ)和肝脏过氧化物酶体增殖物激活受体α(PPARα)的含量。【结果】与正常对照组比较,模型组小鼠具有2型糖尿病特征,并伴有收缩压和血脂异常升高(P<0.05或P<0.01)。在血压调控方面,消渴丸低、高剂量组和消渴丸纯中药组在给药4周后收缩压低于模型组(P<0.05),而格列本脲组收缩压无显著变化(P>0.05);在调节空腹血糖、胰岛素和HOMA-IR方面,给药4周后消渴丸高剂量组与格列本脲组疗效相当(P>0.05),且其调控空腹血糖和HOMA-IR作用均优于消渴丸低剂量组和消渴丸纯中药组(P<0.05);在血脂调控方面,消渴丸低、高剂量组和消渴丸纯中药组TC和LDL-C水平的降低效果与格列本脲组相当(P>0.05)。AngⅡ、PPARα指标结果显示:与模型组比较,消渴丸低、高剂量组和消渴丸纯中药组血清AngⅡ水平均显著降低(P<0.05或P<0.01),而格列本脲组无显著性差异(P>0.05);与模型组比较,消渴丸低、高剂量组,消渴丸纯中药组和格列本脲组肝脏PPARα水平均显著升高(P<0.05或P<0.01),其中,消渴丸高剂量组肝组织PPARα水平最高。【结论】消渴丸对2型糖尿病db/db小鼠不仅具有调控血糖,也有降压和降血脂效果,其疗效是消渴丸纯中药部分和西药格列本脲部分协同作用的结果。消渴丸主要通过格列本脲调控HOMA-IR起到降低血糖的作用,通过消渴丸纯中药部分抑制AngⅡ降低收缩压而实现调控血压,而中药部分与西药部分协同升高肝组织PPARα表Objective To investigate the Xiaoke Pills for hypotensive and hypolipidemic effects on type 2 diabetic db/db mice.Methods By analyzing the regularity of recipe composition,the systolic and diastolic blood pressure,fasting blood glucose,insulin,insulin resistance index(HOMA-IR),serum total cholesterol(TC)and lowdensity lipoprotein cholesterol(LDL-C)in spontaneously diabetic db/db mice were compared in the low-dose Xiaoke Pills group(1.0 g/kg),the high-dose Xiaoke Pills group(4.0 g/kg),the pure Chinese medicine of Xiaoke Pills group(3.996 g/kg)and the Glibenclamide group(4.0 mg/kg)before and after administration.Serum angiotensinⅡ(AngⅡ)and hepatic peroxisome proliferator-activated receptor alpha(PPARα)levels were compared between the groups after treatment.Results Compared with the normal control group,mice in the model group had characteristics of type 2 diabetes mellitus with abnormally elevated systolic blood pressure and lipids(P<0.05 or P<0.01).In terms of blood pressure regulation,the systolic blood pressure in the low-and highdose Xiaoke Pills groups and the pure Chinese medicine of Xiaoke Pills group was lower than that in the model group after 4 weeks of administration(P<0.05),while there was no significant change in systolic blood pressure in the Glibenclamide group(P>0.05).In terms of fasting blood glucose,insulin,HOMA-IR regulation,the effect in the high-dose Xiaoke Pills group was comparable to that in the Glibenclamide group(P>0.05),the effect of fasting blood glucose and HOMA-IR in the high-dose group was superior to that in the low-dose group and the pure Chinese medicine of Xiaoke Pills group(P<0.05).In terms of blood lipid regulation,the effect of TC and LDL-C levels in the low-and high-dose Xiaoke Pills groups and the pure Chinese medicine of Xiaoke Pills group were comparable to that in the Glibenclamide group(P>0.05).The results of AngⅡand PPARαshowed as follows:compared with the model group,the serum AngⅡlevel was significantly decreased in both the low-and high-dose groups of Xiaoke

关 键 词：消渴丸 糖尿病 血压 血脂 血管紧张素Ⅱ(AngⅡ) 过氧化物酶体增殖物激活受体α(PPARα) DB/DB小鼠 

分 类 号：R285.5[医药卫生—中药学]