多效唑与诱抗剂互作对香蕉苗生长及抗枯萎病能力的综合效应  

Comprehensive effects of interaction of paclobutrazol and chemical elicitors on improving banana seedling quality and resistance to Fusarium wilt

作　　者：赵明[1] 邹瑜[1] 龙芳[1] 黄相 莫天利 武鹏[1] 何海旺[1] ZHAO Ming;ZOU Yu;LONG Fang;HUANG Xiang;MO Tian-li;WU Peng;HE Hai-wang(Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]广西农业科学院生物技术研究所,南宁530007

出　　处：《西南农业学报》2023年第5期965-973,共9页Southwest China Journal of Agricultural Sciences

基　　金：广西自然科学基金项目(2021GXNSFBA075045,2022GXNSFAA035543);广西科技重大专项(桂科AA22068090);国家桃产业技术体系(CARS-31)。

摘　　要：【目的】明确多效唑(PP333)与水杨酸(SA)、茉莉酸(JA)、壳聚糖(CTS)和苯并噻二唑(BTH)等药物互作对香蕉苗生长及抗病性的影响,为利用诱抗剂防控香蕉枯萎病(Fusarium wilt of banana)提供参考依据。【方法】以香蕉品种‘宝岛蕉’组培杯苗为试验材料,叶面喷施PP333(A处理)及4种常用诱抗剂单剂(B、C、D和E处理)和双剂(A+B、A+C、A+D和A+E处理),以喷清水为对照(CK),结合伤根浸菌接种法,比较分析各处理香蕉苗的生长状况、抗枯萎病能力和防御酶活性。【结果】处理后30 d,各药物处理香蕉苗的株高均显著低于CK(P<0.05,下同),茎粗总体上高于CK。其中,双剂处理中以PP333配施BTH的株高最低,较CK显著降低31.84%,以PP333配施JA的茎粗最粗,较CK显著增加20.55%。双剂处理香蕉苗的地上部干物质量均显著低于CK,降幅为2.46%~5.74%,根系干物质量较CK显著提高39.02%~43.90%。各药物处理香蕉苗期的枯萎病病情指数均显著低于CK,其中PP333配施BTH和CTS对香蕉枯萎病的诱抗效果较佳,分别达57.63%和50.85%;双剂处理对诱导香蕉苗体内抗性相关酶活性的效应明显,其中,PP333分别配施BTH和CTS,其苯丙氨酸解氨酶(PAL)活性较CK分别提高54.59%~101.69%和42.09%~103.50%,多酚氧化酶(PPO)活性分别提高77.29%~228.77%和66.07%~267.65%,过氧化物酶(POD)活性分别提高121.82%~204.16%和54.31%~117.55%,超氧化物歧化酶(SOD)活性分别提高107.45%~263.51%和110.00%~301.07%,CAT活性分别提高141.95%~442.16%和192.68%~304.70%。【结论】PP333与诱抗剂互作可提高香蕉苗防御酶活性,壮苗效果好,对香蕉枯萎病具有良好的诱抗效果。其中,PP333与BTH和CTS配施的诱导效果更佳,具有生产应用前景。【Objective】The present paper aimed to clarify the effects of the interaction of paclobutrazol(PP333) with salicylic acid(SA),jasmonic acid(JA),chitosan(CTS) and benzothiadiazole(BTH) on the growth and disease resistance of banana seedlings, and provide reference basis for the use of inducers to control Fusarium wilt of banana.【Method】The tissue cultured cup seedlings of banana cultivar ‘Baodao banana' were used as experimental materials, and PP333(treatment A)and four commonly used elicitors(treatment B,C,D and E) and double agent treatments(treatment A+B,A+C,A+D and A+E) were sprayed on the leaves. The growth status, resistance to Fusarium wilt and defense enzyme activities of banana seedlings of each treatment were compared and analyzed by spraying water as the control(CK),combined with the inoculation method of root wounding.【Result】30 days after treatment, the plant height of banana seedlings treated with various drugs was significantly lower than CK(P0.05,the same as below),and the stem diameter was generally higher than CK. Among them, the plant height of PP333 combined with BTH was the lowest in the double agent treatment, which was significantly reduced by 31.84% compared with CK. The stem diameter of PP333 combined with JA was 20.55% significantly higher than CK. The aboveground dry matter quality of banana seedlings treated with double agents was significantly lower than that of CK,with a decrease of 2.46%-5.74%,and the root dry matter quality was significantly increased by 39.02%-43.90% compared with CK. The disease index of banana F. wilt in the seedling stage was significantly lower than that of CK,among which PP333 combined with BTH and CTS had a better effect on inducing resistance to banana F. wilt, reaching 57.63% and 50.85% respectively. The effect of two treatments on the activity of resistance-related enzymes in banana seedlings was obvious. Among them, the activity of phenylalanine ammonia-lyase(PAL) of PP333 combined with BTH and CTS increased by 54.59%-101.69% and 42.09%-103.5

关 键 词：香蕉苗 化学诱抗剂 多效唑 交互作用 壮苗效果 香蕉枯萎病 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]