蓝莓根际土壤微生物群落结构及促生菌筛选  

Microbial community structure and screening of growth-promoting bacteria in blueberry rhizosphere soil

作　　者：王梦姣[1] 郑天骄 WANG Meng-jiao;ZHENG Tian-jiao(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000

出　　处：《西南农业学报》2023年第5期983-991,共9页Southwest China Journal of Agricultural Sciences

基　　金：陕西省科技厅青年项目(2021JQ-755);陕西省高校科学技术协会青年人才托举计划项目(20200206)。

摘　　要：【目的】从促生微生物角度分析蓝莓根际土壤微生态结构,改良蓝莓种植土壤。【方法】利用高通量扩增子测序方法对北高丛蓝莓根际土壤微生物多样性进行分析。利用选择性培养基、比色法等实验方法分析蓝莓根际可培养细菌的促生特性。【结果】土壤样品中有10个细菌门和3个真菌门的相对丰度大于1%,13个细菌属和11个真菌属的相对丰度大于1%;Occallatibacter OTU6、Paraburkholderia OTU3以及Chujaibacter OTU945是细菌OTU相对丰度最高的序列,Pseudogymnoascus OTU1、Fusarium OTU7、Lentinus OTU2是真菌OTU相对丰度最高的序列。蓝莓根际土壤可培养细菌的促生效能分析结果表明,12株蓝莓根际可培养细菌为能够分泌生长素的促生菌,其中L7菌株产生的生长素最多;12株菌均能在解磷培养基上生长,且具有显著解磷圈,其中菌株L42的解磷能力最强;菌株L11和L17具有一定分解硅酸盐的能力;菌株拮抗实验结果表明,L17菌株与L21、L26、L37、L40及L42菌株之间不存在生长抑制作用,菌株L21与菌株L26、L40之间存在强烈抑制作用。16S rDNA测序结果表明,12株促生菌分别属于变形菌门下的布丘氏菌属(Buttiauxella)、伯克霍尔德菌属(Paraburkholderia)以及假单胞菌属(Pseudomonas)。【结论】蓝莓根际微生物群落结构多样性与其他经济作物差异显著,筛选出的促生菌具有良好的解磷效应及生长素产生能力,为后续蓝莓根际微生物菌肥开发提供实验基础。【Objective】The microecological structure of blueberry rhizosphere soil was analyzed from the perspective of growth-promoting microorganisms to improve the blueberry planting soil.【Method】The microbial community structure of blueberry rhizosphere soil was analyzed by high-throughput amplicon sequencing. The growth-promoting characteristics of culturable bacteria in blueberry rhizosphere were analyzed by selective medium and colorimetry.【Result】The relative abundance of 10 bacterial phyla and 3 fungal phyla was greater than 1%, and the relative abundance of 13 bacterial genera and 11 fungal genera was greater than 1%. Occallatibacter OTU6, Paraburkholderia OTU3, and Chujaibacter OTU945 represented the highest numbers of counts more than other bacterial OTU sequences. Pseudogymnoascus OTU1, Fusarium OTU7, and Lentinus OTU2 represented the highest numbers of counts more than other fungal OTU sequences. The growth-promoting characteristics analysis of culturable bacteria in blueberry rhizosphere soil showed that, 12 growth-promoting bacteria that could produce auxin were screened from culturable bacteria in blueberry rhizosphere soil, and L7 strain had the highest auxin production. These 12 growth-promoting bacteria could grow on the phosphorus-solubilizing medium, and had significant phosphorus decomposition halos. Strain L42 had the strongest phosphorus-solubilizing capacity. Strain L11 and L17 could break down silicates.There was no inhibition between L17 strain and L21, L26, L37, L40, L41. But there was strong inhibition between L21 and L26, L40 strains. 16S rDNA sequencing results showed that, these strains were identified as Buttiauxella, Paraburkholderia and Pseudomonas. 【Conclusion】The results indicated that the diversity of microbial community structure in blueberry rhizosphere was significantly different from that of other economic crops, and the growth-promoting bacteria had good phosphorus-solubilizing effect and auxin production capacity, which could provide experimental basis for the subs

关 键 词：根际土壤 蓝莓 群落结构 促生菌 生长素 

分 类 号：S15[农业科学—土壤学]