UPLC-PDA法同时测定柴胡中3种皂苷的含量  

Simultaneous Determination of Three Saponins in Bupleuri Radix by UPLC-PDA

作　　者：马莉 魏娟 付宇[3] MA Li;WEI Juan;FU Yu(Department of Pharmacy,People’s Hospital Affiliated to Hubei University of Medicine,Shiyan 442000,China;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Shiyan 442000,China;People’s Hospital Affiliated to Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]十堰市人民医院(湖北医药学院附属人民医院)药学部,湖北十堰442000 [2]武当特色中药研究湖北省重点实验室,湖北十堰442000 [3]十堰市人民医院(湖北医药学院附属人民医院),湖北十堰442000

出　　处：《食品与药品》2023年第3期221-224,共4页Food and Drug

基　　金：湖北省教育厅自然科学基金项目(编号:B2020110);2018年十堰市科学技术研究与开发项目(编号:18Y72)。

摘　　要：目的建立同时测定柴胡中柴胡皂苷a、柴胡皂苷c、柴胡皂苷d的UPLC方法。方法采用ACQUITY UPLC BEH-C18(2.1 mm×100 mm,1.7μm)色谱柱;以乙腈为流动相A,水为流动相B,梯度洗脱(0~18 min,25%~90%A;18~20 min,90%~90%A);柱温:30℃;流速:0.3 ml/min;检测波长:200 nm;进样量:5μl。结果柴胡中3种成分20 min内实现分离,柴胡皂苷a、柴胡皂苷c和柴胡皂苷d分别在0.65~8.45μg(r=0.9991),0.65~8.45μg(r=0.9995),0.73~9.49μg(r=0.9990)范围内与色谱峰峰面积线性关系良好,加样回收率的分别为99.41%(RSD=0.65%),97.59%(RSD=4.68%),95.55%(RSD=3.28%)。结论此法操作简单,结果准确,重复性好,可用于中药柴胡中3种皂苷类成分的含量测定。Objective To establish a UPLC method for the simultaneous determination of saikosaponin a,saikosaponin c and saikosaponin d.Methods An ACQUITY UPLC BEH-C18(2.1 mm×100 mm,1.7μm)column was used with acetonitrile as mobile phase A and water as mobile phase B for the gradient elution(0-18 min,25%-90%A;18-20 min,90%-90%A).The column temperature was 30℃,the flow rate was 0.3 ml/min,the detection wavelength was 200 nm and the injection volume was 5μl.Results The three kinds of saikosaponins could be separated within 20 minutes by using this method.The linear ranges of saikosaponin a,saikosaponin c and saikosaponin d were 0.65-8.45μg(r=0.9991),0.65-8.45μg(r=0.9995),and 0.73-9.49μg(r=0.99907),respectively.The average recoveries were 99.41%(RSD=0.65%),97.59%(RSD=4.68%)and 95.55%(RSD=3.28%),respectively.Conclusion The method is simple,accurate and reproducible.It can be used for the determination of three kinds of saikosaponins in Bapteuri Radix.

关 键 词：柴胡皂苷A 柴胡皂苷c 柴胡皂苷D UPLC 

分 类 号：R927[医药卫生—药学]