促内皮化β-多肽聚合物修饰的聚氨酯表面抗菌功能研究  

Study on Antibacterial Function of Polyurethane Surface Modified with Endothelializable β-Peptide Polymer

作　　者：周睿毅 佘蕴芮 武月铭 张君宇 王靖[1] 刘润辉[1,2] Rui-yi Zhou;Yun-rui She;Yue-ming Wu;Jun-yu Zhang;Jing Wang;Run-hui Liu(Engineering Research Center for Biomedical Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237;East China University of Science and Technology Shenzhen Research Institute,Shenzhen 518063)

机构地区：[1]华东理工大学材料科学与工程学院教育部医用生物材料工程研究中心,上海200237 [2]华东理工大学深圳研究院,深圳518063

出　　处：《高分子学报》2023年第7期1055-1063,共9页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号21861162010,52203162);上海市优秀青年学术带头人项目(项目号20XD1421400);中国博士后科学基金(基金号2021M701198);博士后创新人才支持计划(项目号BX20220108);华东理工大学深圳研究院自由探索类基础研究项目(项目号2021Szvup042)资助.

摘　　要：由于血管移植物在植入过程中容易引起内皮损伤及微生物感染的风险,迫切需要研究能够促进表面内皮化并抗微生物感染的生物材料来降低这些风险发生的概率.本工作将近期发现的具有促内皮化功能的β-多肽聚合物修饰到人工血管常用材料热塑型聚氨酯(TPU)表面,并研究其抗菌功能.研究表明,β-多肽聚合物修饰表面对耐药革兰氏阳性菌和阴性菌表现出广谱的高效抗菌活性.此外,该功能化TPU表面仍具有优异的选择性促内皮细胞黏附的能力,可满足人工血管移植物抗感染及促内皮化的多功能需求.Vascular grafts are prone to cause risks of endothelial damage and microbial infection during implantation,so there is an urgent need to find multifunctional biomaterials with endothelializable and antibacterial functions to reduce these risks.Host defense peptides(HDPs)have broad-spectrum antimicrobial activity and are widely used to inhibit microbial infections.However,the application of these peptides is limited due to their low stability,expensive and time-consuming synthesis.Therefore,in order to meet the multifunctional requirements of vascular grafts and address these limitations,endothelializableβ-peptide polymer NM_(40)CH_(60) was used as HDPs mimics for antibacterial functional studies in this work.β-Peptide polymer NM_(40)CH_(60) was grafted to the surface of thermoplastic polyurethane(TPU),commonly used in artificial vascular grafts,via a process of O2 plasma activation followed by brominated modification.The effective functionalization of TPU surface was confirmed by ellipsometry,X-ray photoelectron spectroscopy and water contact angle characterization.Antibacterial studies showed that NM40CH60-modified TPU surface exhibited broad-spectrum antibacterial activity against multi-drug resistant Gram-positive and Gram-negative bacteria,as well as efficient contact-killing ability.Antimicrobial mechanism study indicated that NM_(40)CH_(60)-modified TPU surface killed bacteria by interacting with bacterial membrane.Meanwhile,the functionalized TPU surface displayed excellent blood compatibility.In addition,β-peptide polymer NM_(40)CH_(60)-modified TPU surface maintained superior endothelial-selective cell adhesion,which can effectively reduce the risk of microbial infection and endothelial damage of vascular grafts,implying great potential in the treatment of cardiovascular disease.

关 键 词：β-多肽聚合物 宿主防御肽 抗菌 内皮化 

分 类 号：R318.08[医药卫生—生物医学工程]