表达人二肽基肽酶4(hDPP4)转基因小鼠的主要生物学特性指标测定  

Analysis of Biochemical Parameters of Transgenic Mice Expressing Human Dipeptidyl Peptidase 4(hDPP4)

作　　者：隗和儒 翟悦怡 刘超 白天雨 刘树锋 谢英 WEI Heru;ZHAI Yueyi;LIU Chao;BAI Tianyu;LIU Shufeng;XIE Ying(Hebei Key Lab of Laboratory Animal Science,Department of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China;Graduate School,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学实验动物学部,河北省实验动物重点实验室,石家庄050017 [2]河北医科大学研究生学院,石家庄050017

出　　处：《实验动物科学》2023年第3期18-23,共6页Laboratory Animal Science

基　　金：河北省重点研发项目(20277744D)。

摘　　要：目的分析表达人二肽基肽酶4(hDPP4)的转基因小鼠的生物学特性指标,为将其应用于中东呼吸综合征的相关研究提供基础数据。方法本实验室构建了表达hDPP4的转基因小鼠C57BL/6J-TgN(UBC-hDPP4-GFP)CLARS,简称Tg^(hDPP4)。对不同性别(每组5只)的Tg^(hDPP4)小鼠的10项血液生化指标、18项血液生理指标、4~16周龄生长发育指标和8项脏器指数进行测定,并与不同性别(每组5只)的野生型C57BL/6J小鼠进行比较,用统计学方法进行分析。结果与野生型C57BL/6J小鼠相比,Tg^(hDPP4)F_(3)雄鼠的丙氨酸转氨酶和碱性磷酸酶均降低,血糖和血尿氨素均升高;与野生型C57BL/6J小鼠相比,Tg^(hDPP4)F_(3)雌鼠的丙氨酸转氨酶和碱性磷酸酶均升高;Tg^(hDPP4)F_(3)雄鼠的体质量自5周龄开始均高于野生型雄鼠。结论hDPP4的转入对Tg^(hDPP4)小鼠的血糖、血尿氨素、丙氨酸转氨酶、碱性磷酸酶等部分血液生化指标和体质量均产生影响。Objective In order to provide basic data for the application of MERS-CoV studies,biochemical parameters of transgenic mice expressing human dipeptidyl peptidase 4 were examined.Method A transgenic mouse model that expressing hDPP4,named C57BL/6J-TgN(UBC-hDPP4-GFP)CLARS was constructed,which was referred to Tg^(hDPP4).Ten blood biochemical indexes,18 blood physiological indexes,growth and development indexes from 4 to 16 weeks of age and 8 organ indexes of Tg^(hDPP4) mice were measured,and compared with wild-type C57BL/6J mice.Result The alanine aminotransferase and alkaline phosphatase of female Tg^(hDPP4) F_(3) mice was higher than wild-type C57BL/6J mice.The alanine aminotransferase and alkaline phosphatase of male transgenic mice were lower than the wild-type C57BL/6J mice.Elevated blood glucose and blood urea ammonia were found in male Tg^(hDPP4) F_(3) mice.Conclusion Through the analysis of the blood biochemical parameters of Tg^(hDPP4) F_(3) mice,it is found that the exogenous hDPP4 gene can affect the blood sugar,blood urea ammonia,alanine aminotransferase and alkaline phosphatase in transgenic mice and may affect the average body weight of Tg^(hDPP4) mice.

关 键 词：人二肽基肽酶4(hDPP4) 转基因小鼠 血液生化指标 脏器指数 生长发育指标 

分 类 号：Q95-3[生物学—动物学]