自发持续性心房颤动动物实验模型的探索研究  

Experimental Study on Animal Models of Spontaneously Persistent Atrial Fibrillation

作　　者：张冬 张敏[2] 朱瑾彦 李彬[2] ZHANG Dong;ZHANG Min;ZHU Jinyan;LI Bin(Department of Thoracic Surgery,Beijing Jishuitan Hospital,Beijing 100037,China;Chinese Academy of Medical Sciences,Peking Union Medical College,National Center for Cardiovascular Disease,Fuwai Hospital,State Key Laboratory of Cardiovascular Disease,Beijing Key Laboratory of Pre-Clinical Research and Evaluation for Cardiovascular Implant Materials,Animal Experimental Center,Beijing 100037,China)

机构地区：[1]北京积水潭医院,胸外科北京100037 [2]中国医学科学院,北京协和医学院,国家心血管病中心,阜外医院,心血管疾病国家重点实验室,心血管植入材料临床前评价北京市重点实验室,动物实验中心,北京100037

出　　处：《实验动物科学》2023年第3期61-70,共10页Laboratory Animal Science

摘　　要：目的探索建立自发持续性房颤的动物实验模型。方法选择贵州小型猪,经麻醉诱导、呼吸插管后建立二尖瓣返流及假手术组模型。二尖瓣返流模型组4只,对照假手术组4只,对比观察术后最长周期(6个月)内自发持续性房颤的发生与发展,期间(术前、术后即刻、术后3个月、术后6个月)分别进行心电图及心脏超声检测,以便明确房颤及瓣膜返流情况。期间模型若出现自发持续性房颤,实验即刻终止,并进行三维电生理标测;若实验动物模型未出现自发持续性房颤,最长观察时间为6个月,并进行三维电生理标测及房颤易感性检验。结果成功建立二尖瓣返流及假手术组模型,各组最长周期(6个月)内均未出现自发持续性房颤;二尖瓣返流组左心房明显扩大,出现明显结构重构;与对照组相比,二尖瓣返流组短时间房颤片段显著减少,起搏刺激后房颤心率/全部心率比例显著增加;二尖瓣返流组与对照组相比房颤易感性明显增高。结论本实验成功建立持续性房颤的动物实验模型1例,并发现二尖瓣返流可以引起房颤易感性的明显升高。Objective To establish an animal model of spontaneous persistent atrial fibrillation.Method Guizhou miniature pigs were selected and the mitral regurgitation and sham operation group models were established after anesthesia induction and respiratory intubation.Mitral regurgitation model group(4 cases)and sham operation group(4 cases)were compared to observe the occurrence and development of spontaneous persistent atrial fibrillation within the longest postoperative period(6 months).During the period(before surgery,immediately after surgery,3 months after surgery,6 months after surgery),electrocardiogram and cardiac ultrasound were performed respectively,so as to clarify the situation of atrial fibrillation and valve regurgitation.If spontaneous persistent atrial fibrillation appeared in the model during the period,the experiment was immediately terminated and three-dimensional electrophysiological mapping was performed.If there was no spontaneous persistent atrial fibrillation in the experimental animal model,the longest observation time was 6 months,and three-dimensional electrophysiological mapping and susceptibility test of atrial fibrillation were performed.Result The model of mitral regurgitation and sham operation group was established successfully.No spontaneous persistent atrial fibrillation occurred in the longest period(6 months)of each group.In the mitral regurgitation group,the left atrium was obviously enlarged and showed obvious structural remodeling.Compared with the control group,the fraction of short atrial fibrillation in the mitral regurgitation group was significantly reduced,and the ratio of heart rate to total heart rate of atrial fibrillation after pacing stimulation was significantly increased.The mitral regurgitation group was significantly more susceptible to atrial fibrillation than the control group.Conclusion An animal model of persistent atrial fibrillation was successfully established in this study,and it was found that mitral regentation can significantly increase the susceptibilit

关 键 词：心房颤动 二尖瓣返流 动物模型 

分 类 号：Q95-3[生物学—动物学]