黄芪甲苷对1型糖尿病大鼠视网膜病变的影响  

Effect of Astragaloside IV on Type 1 Diabetic Retinopathy in Rats

作　　者：姜帆 孙继超 张佳 武会娟 JIANG Fan;SUN Jichao;ZHANG Jia;WU Huijuan(Beijing Laboratory Animal Research Center,Co.,Ltd.,Beijing 102609,China)

机构地区：[1]北京实验动物研究中心有限公司,北京102609

出　　处：《实验动物科学》2023年第3期92-97,共6页Laboratory Animal Science

摘　　要：目的研究黄芪甲苷对1型糖尿病大鼠视网膜病变的防治效果,探索其作用机制。方法建立1型糖尿病大鼠模型,选取血糖达标大鼠随机分为5组,另设正常对照1组,每组10只。灌胃黄芪甲苷并定期监测血糖、体质量,16周后对各组大鼠视网膜组织的炎性因子IL-6、TNF-α、血清中氧化应激指标MDA和SOD进行检测,对视网膜进行病理分析。结果黄芪甲苷对1型糖尿病大鼠视网膜病变有改善作用。其对血糖和体质量影响较小,但相比于模型对照组,可以显著降低视网膜组织中的IL-6、TNF-α含量和血清中MDA含量(P<0.05),提高血清中的SOD活力(P<0.05),并可降低视网膜内VEGFA的表达。结论黄芪甲苷对1型糖尿病大鼠视网膜病变具有保护作用,作用机制与抑制炎性反应、氧化应激和降低VEGFA表达有关。Objective The purpose of this study was to study the prevention and treatment effect of astragaloside IV on type 1 diabetic retinopathy in rats and explore its mechanism.Method The type 1 diabetic rat model was established.The rats whose blood glucose reached the required level were randomly divided into five groups and a normal control group with ten rats in each group.Astragaloside IV was given by intragastric administration to the rats,their blood glucose and body weight were monitored regularly.After 16 weeks,the levels of inflammatory factors(IL-6,TNF-α)in retinal tissues and oxidative stress indexes(MDA,SOD)in serum were detected,and the retinal pathology was analyzed.Result Astragaloside IV improved type 1 diabetic retinopathy in rats.Compared with the model control group,astragaloside IV significantly decreased the contents of IL-6 and TNF-αin retinal tissue and MDA in serum(P<0.05),increased the activity of SOD in serum(P<0.05),and decreased the expression of VEGFA in retina.Conclusion Astragaloside IV has protective effect on type 1 diabetic rat retinopathy,and its mechanism may be related to inhibition of inflammatory factors,oxidative stress level and VEGFA expression.

关 键 词：黄芪甲苷 糖尿病视网膜病变 氧化应激 炎性因子 

分 类 号：Q95-956[生物学—动物学]