氢气促进人脐静脉内皮细胞增殖和抑制黏附分子释放的机制研究  

Study on the mechanism of hydrogen promoting the proliferation of umbilical vein endothelial cells and inhibiting the release of adhesion molecules

作　　者：刘艳秋[1] 李乐 郭丽伟 张婷玥 张婷婷 吕磊 黄楠[2] 涂秋芬[2] 赵安莎[2] LIU Yanqiu;LI Le;GUO Liwei;ZHANG Tingyue;ZHANG Tingting;LÜLei;HUANG Nan;TU Qiufen;ZHAO Ansha(Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs,School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China;School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China)

机构地区：[1]西南交通大学生命科学与工程学院四川省天然药物仿生合成工程研究中心,四川成都610031 [2]西南交通大学材料科学与工程学院,四川成都610031

出　　处：《华西药学杂志》2023年第3期273-277,共5页West China Journal of Pharmaceutical Sciences

基　　金：四川省国际合作项目(2019YFH0103,2021YFH0056);四川省科技项目(2020YFH0025);中央高校基本科研业务费专项资金(2682022ZTPY045,GX2021160045,2021163,2682021ZTPY002)。

摘　　要：目的探究氢气对脂多糖诱导的人脐静脉内皮细胞(HUVEC)中miR-126及其潜在靶点细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)和E-选择素(E-selectin)表达的影响。方法分4组培养HUVEC,采用不同浓度(0、0.01、0.1、1.0μg·mL^(-1))的脂多糖处理24 h;确定100 ng·mL^(-1)为最佳诱导浓度,建立动脉粥样硬化炎症细胞模型,将细胞分为正常组、模型组、模型+氢气组、模型+阿托伐他汀组;采用CCK-8法检测细胞的存活率,RT-qPCR检测miR-126和黏附分子ICAM-1、VCAM-1、E-selectin在HUVEC中的表达。结果采用100 ng·mL^(-1)脂多糖可成功诱导动脉粥样硬化炎症细胞模型;与模型组比较,氢气可显著促进细胞增殖,上调miR-126的表达,下调ICAM-1、VCAM-1、E-selectin的表达,效果与阿托伐他汀相近。结论氢气可能通过上调miR-126的表达来发挥促进HUVEC增殖和抑制黏附分子释放的作用。OBJECTIVE To explore the effects of hydrogen on the expression of miR-126 and its potential targets intercellular adhesion molecule-1(ICAM-1),vascular cell adhesion molecule-1(VCAM-1)and E-selectin in human umbilical vein endothelial cells(HUVEC)induced by lipopolysaccharide(LPS).METHODS HUVECs were divided into 4 groups and treated with different concentrations of LPS(0,0.01,0.1,1,0μg·mL^(-1))for 24 hours.The induced concentration of 100 ng·mL^(-1) was determined to establish an atherosclerosis(AS)inflammatory cell model.HUVECs were divided into:control group,LPS model group,and LPS+hydrogen group,and LPS+atorvastatin group.The cell survival rate was determined by CCK-8 assay,and the expression of miR-126 and adhesion molecules ICAM-1,VCAM-1 and E-selectin in HUVEC was detected by RT-qPCR.RESULTS 100 ng·mL-1 of LPS can successfully induce the AS inflammatory cell model.Compared with the model group,hydrogen could promote cell proliferation,up-regulate the expression of miR-126 and down-regulate the expression of ICAM-1,VCAM-1,and E-selectin.CONCLUSION Hydrogen may promote the proliferation of HUVEC and inhibit the release of adhesion molecules by up-regulating the expression of miR-126.

关 键 词：动脉粥样硬化 细胞炎症模型 脂多糖诱导 富氢培养基 MIR-126 黏附分子 细胞间黏附分子-1 血管细胞黏附分子-1 E-选择素 作用机制 

分 类 号：R96[医药卫生—药理学]