TLR-2、TNF-α与输卵管妊娠的相关性研究  

Correlation between Toll-like receptor 2 and tumor necrosis factorαand fallopian tube pregnancy

作　　者：徐海花 林冠 孙蓬明[1] Xu Haihua;Lin Guan;Sun Pengming(Department of Obstetrics and Cynecology,Fujian Maternal and Child Health Hospital,Affiliated Hospital of Fujian Medical University,Fuzhou Fujian 350001,P.R.China)

机构地区：[1]福建省妇幼保健院福建医科大学附属医院妇产科,福建福州350001

出　　处：《中国计划生育和妇产科》2023年第6期34-36,共3页Chinese Journal of Family Planning & Gynecotokology

基　　金：福建省妇幼保健院科技创新启动基金(项目编号:17-21)。

摘　　要：目的探讨在妊娠状态下的输卵管组织中Toll样受体2(Toll-like receptor 2,TLR-2)的表达、血清肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的浓度及其与输卵管妊娠的关系。方法选择2017年10月至2018年3月福建省妇幼保健院收治的30例输卵管壶腹部妊娠的患者为输卵管妊娠组,其输卵管组织分为胚胎种植部位(壶腹部)、胚胎非种植部位(峡部);同期30例足月妊娠孕妇为宫内妊娠组,其输卵管组织为输卵管峡部。采用ELISA法检测两组外周血血清中TNF-α浓度;逆转录RT-PCR法检测输卵管组织中TLR-2 mRNA表达水平。结果输卵管妊娠组的外周血血清TNF-α[106.12(64.38-185.40)pg/mL]较宫内妊娠组[64.16(45.37-77.42)pg/mL]浓度更高;输卵管妊娠组的TLR-2 mRNA[2.98(1.67-4.24)]较宫内妊娠组[2.10(1.12-3.45)]表达更高,差异均有统计学意义(P<0.05);TLR-2 mRNA在输卵管胚胎种植部位[1.68(1.59-2.71)]中的表达较非种植部位[4.23(3.32-5.03)]低,差异有统计学意义(P<0.05)。结论输卵管组织部位TLR-2 mRNA的表达和血清TNF-α与输卵管妊娠相关,且TLR-2 mRNA在输卵管胚胎非种植部位的表达更高,TLR-2、TNF-α有望助力于输卵管妊娠的早期诊断。Objective To investigate the expression of Toll-like receptor 2(TLR-2)in tubal tissue and the concentration of serum tumor necrosis factor-α(TNF-α)in tubal pregnancy and their relationship with tubal pregnancy.Methods 30 cases of tubal ampullary pregnancy admitted to Fujian Maternal and Child Health Hospital from October 2017 to March 2018 were selected as tubal pregnancy group,the tubal tissues were divided into embryo implantation site(ampulla)and embryo non-implantation site(isthmus).In the same period,30 cases of full-term pregnant women were trauterine pregnancy group,and the tubal tissues were the isthmus of fallopian tube.The concentration of TNF-αin peripheral blood serum of the two groups was detected by ELISA.The expression of TLR-2 mRNA in fallopian tube tissues was detected by reverse transcription RT-PCR.Results The concentration of TNF-αin the peripheral blood serum of the tubal pregnancy group[106.12(64.38-185.40)pg/mL]was higher than that of the intrauterine pregnancy group[64.16(45.37-77.42)pg/mL],the expression of TLR-2 mRNA in tubal pregnancy group[2.98(1.67-4.241)]as higher than that in intrauterine pregnancy group[2.10(1.12-3.45)],and the differences were statistically significant(P<0.05).The expression of TLR-2 mRNA in the implantation site[1.68(1.59-2.71)]was significantly lower than that in the non-implantation site[4.23(3.32-5.03)],and the difference was statistically significant(P<0.05).Conclusionss The expression of TLR-2 mRNA in tubal tissue and serum TNF-αare related to tubal pregnancy,and the expression of TLR-2 mRNA is higher in non-implantation site of tubal embryos.TLR-2,TNF-αare expected to contribute to the early diagnosis of tubal pregnancy.

关 键 词：输卵管妊娠 TLR-2 mRNA TNF-Α 

分 类 号：R714.221[医药卫生—妇产科学]