羟胺对硝化菌活性及其动力学参数的影响  

Effects of hydroxylamine on the activity and kinetic parameters of nitrifying bacteria

作　　者：曾天续 张永显 严渊 刘宏 马娇[1,2,3] 党鸿钟 吴新波 李维维 陈永志[1,2,3] ZENG Tianxu;ZHANG Yongxian;YAN Yuan;LIU Hong;MA Jiao;DANG Hongzhong;WU Xinbo;LI Weiwei;CHEN Yongzhi(Key Laboratory of Yellow River Water Environment in Gansu Province,Lanzhou Jiaotong University,Lanzhou 730070,Gansu,China;School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,Gansu,China;Technical Center of Sewage Treatment Industry in Gansu Province,Lanzhou 730070,Gansu,China;Gansu Research Institute of Light Industry Company,Limited,Lanzhou 730000,Gansu,China)

机构地区：[1]兰州交通大学甘肃省黄河水环境重点实验室,甘肃兰州730070 [2]兰州交通大学环境与市政工程学院,甘肃兰州730070 [3]甘肃省污水处理行业技术中心,甘肃兰州730070 [4]甘肃省轻工研究院有限责任公司,甘肃兰州730000

出　　处：《化工进展》2023年第6期3272-3280,共9页Chemical Industry and Engineering Progress

基　　金：甘肃省科技计划(20JR2RA002);甘肃省黄河水环境重点实验室开放基金(21YRWEK006);甘肃省高等学校产业支撑计划(2020C-38);兰州交通大学天佑创新团队(TY2020XX);兰州市科技计划(2021-1-180);甘肃省青年科技基金计划(20JR5RA075)。

摘　　要：采用序批式间歇反应器(SBR)处理模拟生活污水,研究羟胺(NH_(2)OH)对硝化菌活性、动力学参数及其对氨氧化菌(AOB)抑制后,AOB活性恢复的影响。批次试验结果表明,当NH_(2)OH浓度为4.5mg/L时,AOB活性最大,亚硝酸盐氧化菌(NOB)活性减小,并且NO_(2)^(-)-N氧化速率(Q^(NOB)_(max))减小至8.00mg/(L·h),NO_(2)^(-)-N半饱和常数(K^(NOB)_(NO_(2)))增大至7.77mg/L,表明NH_(2)OH对NOB的抑制类型为混合抑制。长期试验R1、R2以缺氧/好氧模式运行,R3以好氧/缺氧交替4次运行,R1不投加NH_(2)OH,R2、R3投加NH_(2)OH。结果表明,R1中全程无亚硝积累。在R2中投加4.5mg/L NH_(2)OH,AOB活性为(6.04±0.4)mg N/(g MLVSS·h)。当NH_(2)OH浓度为10mg/L时,AOB和NOB的活性分别下降至(0.16±0.1)mg N/(g MLVSS·h)和(0.15±0.1)mg N/(g MLVSS·h),之后在高曝气且不投加NH_(2)OH的条件下,AOB活性在第5天恢复。在R3中投加4.5mg/L NH_(2)OH,第7天出水NO_(2)^(-)-N/NH_(4)^(+)-N为1.2。16S r RNA高通量测序表明,添加4.5mg/L NH_(2)OH后,R2和R3中AOB、NOB的相对丰度分别为6.6%、1.3%和3.0%、1.9%,AOB菌属为Ellin6067和Nitrosomonas,NOB菌属为Unidentified Nitrospiraceae。The effects of hydroxylamine(NH_(2)OH)on the activity and kinetic parameters of ammonia oxidizing bacteria(AOB)and the recovery of AOB activity after inhibition were investigated using SBR to treat synthetic domestic sewage.Results of batch test showed that when the concentration of NH_(2)OH was 4.5mg/L,the activity of AOB showed the highest level,the activity of nitrite oxidizing bacteria(NOB)decreased,and the oxidation rate of NO_(2)^(-)-N(Q^(NOB)_(max))decreased to 8.00mg/(L·h).The half-saturation constant of NO_(2)^(-)-N(K^(NOB)_(NO_(2)))increased to 7.77mg/L,indicating that the inhibition type of NH_(2)OH on NOB was mixed inhibition.In the long-term experiments,R1 and R2 were operated in anoxic/aerobic,and R3 was operated in aerobic/anoxic mode four times alternately per day.NH_(2)OH was added into R2 and R3 reactors and not in R1.The results showed that,there was no nitrite accumulation in R1.When the concentration of NH_(2)OH was 4.5mg/L in R2,the activity of AOB was(6.04±0.4)mgN/(gMLVSS·h)on the 6th day.When the concentration of NH_(2)OH was 10mg/L,the activities of AOB and NOB decreased to(0.16±0.1)mgN/(gMLVSS·h)and(0.15±0.1)mgN/(gMLVSS·h),respectively.The activities of AOB recovered on the 5th day under high strength aeration without adding NH_(2)OH.When the concentration of NH_(2)OH was 4.5mg/L in R3,the ratio of NO_(2)^(-)-N/NH_(4)^(+)-N in effluent was 1.2 on the 7th day.Highthroughput sequencing of 16S rRNA showed that the relative abundance in R2 and R3 of AOB and NOB were 6.6%,1.3%,and 3.0%,1.9% when the concentration of NH_(2)OH was 4.5mg/L.Ellin 6067 and Nitrosomonas belonged to AOB,and Unidentified Nitro spiraceae belonged to NOB.

关 键 词：羟胺 氨氧化菌 亚硝酸盐氧化菌 短程硝化 AOB活性恢复 动力学参数 

分 类 号：X703.1[环境科学与工程—环境工程]