基于超高效液相色谱与红外光谱的金银花药材产地溯源  

Geographic Origin Tracing of Lonicera Japonica by FTIR and UPLC

作　　者：李籽杉 杜泽飞 杨晓 丁丰 夏从龙 周萍 段宝忠 LI Zishan;DU Zefei;YANG Xiao;DING Feng;XIA Conglong;ZHOU Ping;DUAN Baozhong(College of Pharmaceutical Science,Dali University,Dali 671000,China;Yunnan Beihua Agricultural Development Co.,Ltd,Yunlong 672700,China)

机构地区：[1]大理大学药学院,大理671000 [2]云南省北花农业发展有限公司,云龙672700

出　　处：《医药导报》2023年第7期1067-1073,共7页Herald of Medicine

基　　金：云南省重大科技专项(202002AA100007);云南省院士专家工作站(202105AF150053)。

摘　　要：目的建立金银花产地溯源方法。方法采用傅里叶变换红外光谱(FTIR)与超高效液相色谱(UPLC)法,建立山东、云南产区不同品种金银花样品的指纹图谱;采用主成分分析、层次聚类分析和相似度分析对样品产地溯源进行研究。结果不同产区金银花样品UPLC指纹图谱相似度>0.9,质量均一;基于UPLC色谱可实现不同产区金银花产地溯源,但无法识别同一产区不同品种样品;基于FTIR光谱可实现金银花品种和产地溯源。结论基于光谱和色谱指纹图谱方法结合化学计量学手段,可为金银花药材的产地和品种溯源提供技术支撑。Objective To establish a method for tracing the origins of Lonicera japonica.Methods Fourier transform infrared(FTIR)and ultra-high performance liquid chromatography(UPLC)were used to establish fingerprints of L.japonica of different varieties in Shandong and Yunnan Province.The samples'geographical origin was investigated using FTIR and UPLC combined with pattern recognition analysis,including similarity analysis,principal component analysis(PCA),and hierarchical cluster analysis(HCA).Results The similarities of UPLC fingerprints were greater than 0.9,which suggested that the quality of these samples was uniform.Moreover,the UPLC fingerprint can discriminate the origins of different individuals from various sources.But it cannot distinguish the other L.japonic varieties from the same place.Furthermore,FTIR was applied to trace the origins and varieties of L.japonica,and satisfactory results were achieved.Conclusion The method based on FTIR,UPLC fingerprints,and chemometrics in this paper can provide a technical basis for tracing the source of L.japonica from different places.

关 键 词：金银花 超高效液相色谱法 傅里叶变换红外光谱 化学计量学 产地溯源 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]