Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets  

作　　者：Zhenfu Wang Jie Gao Qinghong Shi Xiaoyan Dong Yan Sun 

机构地区：[1]Department of Biochemical Engineering,School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education),Tianjin University,Tianjin 300350,China

出　　处：《Chinese Journal of Chemical Engineering》2023年第4期119-125,共7页中国化学工程学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2021YFC2102801);the National Natural Science Foundation of China(21621004)。

摘　　要：Oriented immobilization of enzymes helps to maintain their native structure and proper orientation for high-performance engineering to meet extensive biocatalysis demands.However,the supporting materials used for orientated immobilization are usually costly or complicated in preparation,affecting their practical applications.In this work,a facile purification and immobilization method was proposed for enzyme immobilization based on organic-inorganic hybrid calcium phosphate nanocrystal(Ca Ps)induced by Cu^(2+) modified bovine serum albumin(BSA-Cu).Then,the as-prepared hybrid calcium phosphate nanosheet,BSA-Cu@Ca Ps,was utilized for one-pot purification and immobilization of His-tagged organophosphorus hydrolase(OPH)by metal-affinity binding to the incorporated BSA.BSA-Cu@Ca PsOPH exhibited enhanced p H stability and thermal stability compared to the free enzyme.Moreover,BSA-Cu@Ca Ps-OPH could retain more than 75%and 56%of initial activity after reuse 5 and 10 times,respectively.The results demonstrated that this facile strategy was promising for the effective biodegradation of organophosphorus pesticides with the immobilized enzyme.

关 键 词：Calcium phosphate nanocrystal(CaPs) Biomimetic mineralization Organophosphorus hydrolase(OPH) Oriented immobilization HYDROLYSIS Methyl parathion 

分 类 号：TQ426.97[化学工程] TB383.1[一般工业技术—材料科学与工程]