宏基因组第二代测序技术在神经外科颅内感染病原学诊断中的应用价值  

Application of the second generation of macrogenome sequencing technology in the etiology diagnosis of intracranial infection in neurosurgery

作　　者：于海东[1] 朱东[1] 高麟[1] 李海龙[1] Yu Haidong;Zhu Dong;Gao Lin;Li Hailong(The Second Affiliated Hospital of Baotou Medical College,Baotou,Neimenggu 014030,China)

机构地区：[1]包头医学院第二附属医院,内蒙古包头014030

出　　处：《首都食品与医药》2023年第12期44-45,共2页Capital Food Medicine

摘　　要：目的分析宏基因组第二代测序技术在神经外科颅内感染病原学诊断中的应用价值。方法选择包头医学院第二附属医院2020年1月-2022年1月收治的18例神经外科手术后拟诊颅内感染患者为研究对象,两周后由3位神经外科专家共同判断是否确诊为颅内感染。18例患者均进行常规脑脊液细菌培养和宏基因组第二代测序,将宏基因组二代测序技术检测结果和脑脊液细菌培养结果进行对比。结果宏基因组第二代测序技术检测诊断神经外科术后颅内感染的灵敏度和特异度及准确率分别为100%(12/12)、83.3%(5/6)、94.4%(17/18);脑脊液细菌培养组灵敏度和特异度及准确率分别为66.7%(8/12)、50.0%(3/6)、61.1%(11/18);宏基因组第二代测序技术灵敏度、特异度、准确率均高于脑脊液细菌培养组,差异有统计学意义(P<0.05)。结论宏基因组第二代测序技术在神经外科颅内感染病原学诊断中具有一定价值,值得临床重视并推广。Objective To analyze the application of the second generation macro-genome sequencing technology in the etiology diagnosis of intracranial infection in neurosurgery.Methods 18 patients with intracranial infection after neurosurgery were selected from January 2020 to January 2022.After two weeks,three neurosurgery experts jointly judged whether the diagnosis was intracranial infection.Nine cases of intracranial infection were confirmed by gold standard cerebrospinal fluid bacterial culture.The second generation of macrogenome sequencing technology was performed in 18 patients,and the detection results were compared with cerebrospinal fluid bacterial culture.Results The sensitivity,specificity and accuracy of mNGS in the diagnosis of intracranial infection after neurosurgery were 100%(12/12),83.3%(5/6)and 94.4%(17/18),respectively;the sensitivity,specificity and accuracy of bacterial culture group were 66.7%(8/12),50.0%(3/6)and 61.1%(11/18)respectively;the sensitivity,specificity and accuracy of the second generation of macrogenome sequencing technology were higher than those of the conventional cerebrospinal fluid bacterial culture group,and the difference was statistically significant(P<0.05).Conclusion The second generation sequencing technology of macrogenomes has certain value in the etiology diagnosis of intracranial infection in neurosurgery,which is worthy of attention and promotion.

关 键 词：宏基因组第二代测序技术 神经外科颅内感染 脑脊液细菌培养 

分 类 号：R651.1[医药卫生—外科学]