Regulation of A 9-cis-epoxycarotenoid Dioxygenase(NCED)Gene from Raspberry on High Salinity and Cold Tolerance  

作　　者：Yang Guo-hui Li Bo-lin Wang Ze-min Yu Hang Wang Jia-ming Li Tie-mei 

机构地区：[1]The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions/College of Horticulture&Landscape Architecture,Northeast Agricultural University,Harbin 150030,China [2]Basic Teaching Department,Heilongjiang University of Industry and Business,Harbin 150025,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2023年第2期32-42,共11页东北农业大学学报（英文版）

基　　金：Supported by the Postdoctoral Scientific Research Development Fund of Heilongjiang Province,China(LBH-Z21119);the Natural Science Fund Joint Guidance Project of Heilongjiang Province(LH2020C009);Young Talent Project of Northeast Agricultural University(19QC06)。

摘　　要：Abscisic acid(ABA)is a plant hormone that plays an important role in plant development and abiotic stress.The 9-cis-epoxycarotenoid dioxygenase(NCED)is a key rate-limiting enzyme in the ABA biosynthetic pathway.The physiological and molecular mechanisms of NCED regulating plant development and abiotic stress tolerance have been reported in many plant species,but gene function of RiNCEDs in Rubus idaeus L.is rarely reported.In this study,the open reading frame(ORF)sequence of RiNCED2 in red raspberry fruit was isolated and the function of this gene under abiotic stress was investigated.While RiNCED2 was induced by cold,high salinity,drought and ABA,it was highly expressed in new leaves as measured by real-time qPCR.Overexpression of RiNCED2 in Arabidopsis under both high-salt and cold stress increased ABA content,demonstrating that RiNCED2 was involved in ABA biosynthesis.Meanwhile,the leaf wilting degree of transgenic Arabidopsis was less,while the content of malondialdehyde(MDA)was significantly reduced,and the chlorophyll content,proline content,peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)activities were significantly increased.These results indicated that overexpression of RiNCED2 enhanced the resistance of transgenic Arabidopsis to high salt and cold.

关 键 词：9-cis-epoxycarotenoid dioxygenase RASPBERRY high salt cold 

分 类 号：S663.2[农业科学—果树学]