孩儿参多糖对小鼠特异性皮炎的修复作用  

Improvement effects of Pseudostellaria heterophylla(Miq.)Pax.polysaccharide on atopic dermatitis mice

作　　者：刘晓英 韩萍 太美灵 朱溶滔 杜志云 LIU Xiaoying;HAN Ping;TAI Meiling;ZHU Rongtao;DU Zhiyun(Infinitus(China)Company Ltd,Jiangmen,Guangdong 529000,China;School of Biomedical and Pharmaceutical Sciences,Guangdong University of Technology,Guangzhou,Guangdong 510006,China;Allan Conney Biotechnology Company Ltd,Foshan,Guangdong 528200,China)

机构地区：[1]无限极(中国)有限公司,广东江门529000 [2]广东工业大学生物医药学院,广东广州510006 [3]佛山市康伲爱伦生物技术有限公司,广东佛山528200

出　　处：《湖南农业大学学报（自然科学版）》2023年第3期366-370,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(21272043)。

摘　　要：采用脂多糖(LPS)诱导的巨噬细胞RAW264.7模型检测孩儿参多糖调控NO水平;构建2,4–二硝基氟苯诱导的小鼠特异性皮炎模型,检测小鼠的皮损情况、组织病理变化及皮肤组织中丝聚蛋白(FLG)、紧密连接蛋白(CLDN1)、白介素–4(IL–4)、白介素–13(IL–13)和干扰素–γ(INF–γ)的含量,探讨孩儿参多糖对小鼠特异性皮炎的改善作用及调控机制。结果显示:制备的孩儿参多糖可显著抑制LPS诱导的RAW264.7细胞分泌NO;外用给予孩儿参多糖处理后,特异性皮炎小鼠的皮损症状明显改善,经皮失水率降低,表皮增生现象减轻,FLG和CLDN1含量增加,炎症因子IL–4、IL–13和INF–γ的表达水平降低。可见,孩儿参多糖可改善皮肤屏障和降低炎症因子分泌,发挥修复小鼠特异性皮炎的作用。In this study,with an aim to understand the improvement effect and regulatory mechanism of Pseudostellaria heterophylla(Miq.)Pax.polysaccharides on atopic dermatitis mice,the NO levels regulated by P.heterophylla polysaccharides was detected using a macrophage RAW264.7 model induced by LPS,and the atopic dermatitis model in mice was constructed by 2,4-dinitrofluorobenzene and the skin lesions,pathological changes were collected and the content of filaggrin(FLG),claudin-1(CLDN1),interleukin-4(IL-4),interleukin-13(IL-13)and interferon-γ(INF-γ)in skin tissue was measured using ELISA method.According to the results,P.heterophylla polysaccharide prepared in this study could significantly inhibit NO secretion in RAW264.7 cells induced by LPS.Under external treatment with P.heterophylla polysaccharide,the DNFB-induced atopic dermatitis skin symptoms of mice was alleviated,both the rate of transdermal water loss and the thickness of the epidermal layer were reduced,meanwhile,the contents of FLG and CLDN1 proteins were increased and the inflammatory factors IL-4,IL-13 and INF-γwere inhibited.It could be concluded that P.heterophylla polysaccharides could improve the skin barrier and reduce the secretion of inflammatory cytokines,playing a significant role in repairing atopic dermatitis in mice.

关 键 词：小鼠 特异性皮炎 孩儿参多糖 免疫调控 皮肤屏障 炎症因子 

分 类 号：R285.5[医药卫生—中药学]