基于转录组学策略分析川银花对耐药铜绿假单胞菌生物被膜的影响  

Effect of Lonicerae Similis Flos on Biofilm of Drug-Resistant Pseudomonas Aeruginosa Based on Transcriptomics Strategy

作　　者：赵玉婷 廖黎 王镜雅 杨晨 鲁兰 ZHAO Yuting;LIAO Li;WANG Jingya;YANG Chen;LU Lan(Sichuan Industrial Institute of Antibiotics,Chengdu University,Chengdu 610052,China)

机构地区：[1]成都大学四川抗菌素工业研究所,四川成都610052

出　　处：《成都大学学报（自然科学版）》2023年第2期119-124,共6页Journal of Chengdu University（Natural Science Edition）

基　　金：国家自然科学基金项目(81803812);四川省科技厅科技创新苗子工程项目(2022046)。

摘　　要：采用结晶紫染色法、扫描电镜法观察亚抑菌浓度川银花水煎液对耐药铜绿假单胞菌生物被膜形成的影响,并从整体水平上运用转录组学技术分析耐药铜绿假单胞菌基因表达情况,对差异基因进行功能富集及通路分析.结果显示,转录组测序共筛选出362个差异表达基因,其中下调基因206个,上调基因156个,可为耐药铜绿假单胞菌在临床上感染造成的相关疾病的治疗提供新思路.This study investigated the effect of sub-MICs of Lonicerae similis flos decoctions on the formation of biofilm of clinical drug-resistance pseudomonas aeruginosa(PA).Crystal violet staining and scanning by electron microscopy were applied to observe the effect.And transcriptomics was used to analyze the gene expression of drug-resistance PA at the overall level,and analyze the function enrichment and pathway of these genes.A total of 362 differentially expressed genes were screened by transcriptome sequencing,including 206 down regulated genes and 156 up-regulated genes so as to explore the potential mechanism of sub-MICs of Lonicerae similis flos decoctions inhibiting the biofilm of clinical drug-resistance PA and provide new ideas and a series of references for the treatment of related diseases caused by clinical infection of drug-resistant pseudomonas aeruginosa.

关 键 词：川银花 转录组学 铜绿假单胞菌 亚抑菌浓度 生物被膜 

分 类 号：R965[医药卫生—药理学]