K^(+)、Mg^(2+)诱导下解淀粉芽孢杆菌A3代谢物响应研究  

Study on Metabolite Response of Bacillus amyloliquefaciens A3 under K^(+)and Mg^(2+)Induction

作　　者：敖静[1] 李杨[1] 高晓梅[1] 刘晓辉[1] 孙玉禄[1] AO Jing*;LI Yang;GAO Xiaomei;LIU Xiaohui;SUN Yulu(Microbial Research Institute of Liaoning Province,Chaoyang 122000,Liaoning,China)

机构地区：[1]辽宁省微生物科学研究院,辽宁朝阳122000

出　　处：《四川农业大学学报》2023年第3期488-494,共7页Journal of Sichuan Agricultural University

基　　金：辽宁省“兴辽英才计划”项目(XLYC2002048);辽宁省农科院土壤微生态学科建设项目(2019DD154522);辽宁省微生物科学研究院自主创新科研项目(2021-02)。

摘　　要：【目的】研究K^(+)和Mg^(2+)诱导对解淀粉芽孢杆菌(Bacillus amyloliquefaciens)A3的代谢的影响。【方法】以A3发酵液为研究对象,利用非靶向代谢组学和多元统计分析方法对发酵液中差异代谢物进行筛选,通过KEGG数据库对代谢物进行通路富集分析。【结果】K^(+)和Mg^(2+)的添加对发酵液中代谢物有显著影响,主要差异代谢物包括肽类和有机酸类等小分子化合物;相较于空白发酵液,K^(+)处理组检测到120个差异代谢物,其中80个代谢物显示为上调,如精胺、腺嘌呤和L-组氨酸等;Mg^(2+)处理组检测到162个差异代谢物,其中51个代谢物显示为上调,如尿嘧啶、烟酰胺,111个代谢物下调,主要包括L-谷氨酸、L-天冬氨酸等氨基酸类化合物。KEGG数据库共富集到115调代谢通路,主要包括代谢和生物合成途径,其中显著富集通路K^(+)处理组54条,如泛酸盐和辅酶A的生物合成、辅因子的生物合成;Mg^(2+)处理组29条,如氨基酰基-tRNA的生物合成等。【结论】K^(+)的添加提高了A3的细胞代谢水平;K^(+)和Mg^(2+)均可间接导致细胞中NAD/NADP的增加,同时可能使A3的细胞能量代谢也发生了变化;Mg^(2+)的添加对A3胞内的蛋白质合成和降解产生干扰。【Objective】This study purposes to study the effects of K^(+)and Mg^(2+)induction on the metabolism of Bacillus amyloliquefaciens A3.【Method】Taking A3 fermentation broth as the research object,this study employed non-targeted metabolomics and multivariate statistical analysis to screen the differential metabolites in the fermentation broth.Pathway enrichment analysis of metabolites was performed by KEGG database.【Result】The results showed that the addition of K^(+)and Mg^(2+)had significant effects on metabolites in the fermentation broth.The main differential metabolites included peptides,organic acids and other small molecular compounds.Compared with the blank fermentation broth,120 differential metabolites were detected in the K^(+)treatment group,among which 80 metabolites were up-regulated.Such as spermine,adenine,L-histidine and so on.In Mg^(2+)treatment group,162 differential metabolites were detected,among which 51 metabolites were up-regulated,such as uracil and nicotinamide,and 111 metabolites were down-regulated,mainly including amino acid compounds such as L-glutamate and Laspartic acid.A total of 115 regulated metabolic pathways were enriched in KEGG database,mainly including metabolic and biosynthetic pathways.Among them,54 pathways were significantly enriched in K^(+)treatment group,such as the biosynthesis of pantothenate and coenzyme A,and the biosynthesis of cofactors;Mg^(2+)treatment group 29,such as aminoacyl-tRNA biosynthesis.【Conclusion】The addition of K^(+)increased the cellular metabolic level of A3.Both K^(+)and Mg^(2+)can indirectly lead to the increase of NAD/NADP in cells,and may also change the energy metabolism of A3 cells.The addition of Mg^(2+)interfered of NAD/NADP in cells,and may also change the energy metabolism of A3 cells.The addition of Mg2+interfered with protein synthesis and degradation in A3 cells.

关 键 词：解淀粉芽孢杆菌 非靶向代谢组学 多元统计分析 代谢物 

分 类 号：S182[农业科学—农业基础科学]