藏猪心肌细胞对低氧环境的适应性特征  

Adaptive Characteristics of Tibetan Pig's Cardiomyocytes to Hypoxic Environment

作　　者：杨麒天 吴生瑞 杨春旭 杨雅楠[1] 蔡原[1] YANG Qitian;WU Shengrui;YANG Chunxu;YANG Yanan;CAI Yuan(Gansu Agricultural University,College of Animal Science and Technology,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《四川农业大学学报》2023年第3期509-514,545,共7页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金地区资助项目(32060730);甘肃农业大学青年导师扶持基金资助项目(GAU-QDFC-2018-05);国家级大学生创新创业训练计划(202010733015,SRTP藏猪心肌细胞缺氧/复氧模型的研究)。

摘　　要：【目的】探究藏猪心肌细胞对低氧环境的适应性特征。【方法】分离、鉴定藏猪和长白猪的心肌细胞并置于低氧(2%O_(2))和常氧(21%O_(2))环境下培养,观察分析不同时间对心肌细胞形态和增殖率变化的影响趋势。【结果】通过优化心肌细胞培养方法发现,在胰酶消化20 min并且选用CMM培养基时细胞贴壁生长良好,排列均匀,悬浮细胞较少。低氧培养时,心肌细胞呈现明显的纤维化且出现凝集性变化特征;培养72 h时,长白猪的心肌细胞纤维化和凝集性变化较藏猪更明显。低氧环境下培养藏猪和长白猪心肌细胞6 h时,藏猪的增殖率高于长白猪(42%),有助于其更好地适应低氧环境。【结论】藏猪心肌细胞适应低氧环境的能力明显高于长白猪心肌细胞,并且藏猪的心肌细胞所受损伤相较于长白猪心肌细胞明显较小。研究可为进一步揭示藏猪心肌细胞响应低氧机制提供参考,并为平原猪种引入高原低氧地区提供理论支持。【Objective】This paper purposes to investigate the adaptability of Tibetan pig's cardiomyocytes to hypoxic environment.【Method】Cardiomyocytes of Tibetan pigs and Landrace pigs were cultured in hypoxic(2%O2)and normoxic(21%O2)environments for different times to observe and analyze the cellular morphology and the trend of proliferation rate which were isolated and identified.【Result】Though optimizing cardiomyocyte culture methods,it was found that when select CMM medium and trypsin enzyme digest for 20 min,the cells had better adherent growth,uniform arrangement and fewer suspended cells.The cardiomyocytes showed fibrosis and aggregation,and the changes were more obvious when cultured in hypoxia environment for 72 h.In addition,cardiomyocytes of Tibetan pigs and Landrace pigs were cultured with hypoxic concentration until the sixth hour,the proliferation rate of cardiomyocytes of Tibetan pigs was higher than that of Landrace pigs(42%),which may help Tibetan pigs adapt better to the hypoxic environment.【Conclusion】The ability of Tibetan pig's cardiomyocytes to adapt to the low oxygen environment is significantly higher than that of Changbai pigs,and the damage of cardiomyocytes of Tibetan pigs was significantly minor compared to that of Changbai pigs.This study can provide reference for understanding the responding mechanism of Tibetan pig's cardiomyocytes in response to hypoxia,and provide theoretical support for the introduction of other plain pig breeds to hypoxia areas in the plateau.

关 键 词：藏猪 心肌细胞 低氧适应 增殖率 

分 类 号：S828.89[农业科学—畜牧学]