A novel bat coronavirus with a polybasic furin-like cleavage site  

作　　者：Wentao Zhu Yuyuan Huang Jian Gong Lingzhi Dong Xiaojie Yu Haiyun Chen Dandan Li Libo Zhou Jing Yang Shan Lu 

机构地区：[1]Department of Infectious Diseases and Clinical Microbiology,Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital,Capital Medical University,Beijing,100020,China [2]State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China [3]Hainan Provincial Center for Disease Control and Prevention,Haikou,570203,China [4]Research Units of Discovery of Unknown Bacteria and Function,Chinese Academy of Medical Sciences,Beijing,100730,China

出　　处：《Virologica Sinica》2023年第3期344-350,共7页中国病毒学（英文版）

基　　金：the National Key R&D Pro-gram of China(2019YFC1200501);Research Units of Discovery of Un-known Bacteria and Function(2018RU010);the Golden Seed Program of Beijing Chaoyang Hospital(CYJZ202220).

摘　　要：The current pandemic of COVID-19 caused by a novel coronavirus,severe acute respiratory syndrome coronavirus-2(SARS-CoV-2),threatens human health around the world.Of particular concern is that bats are recognized as one of the most potential natural hosts of SARS-CoV-2;however,coronavirus ecology in bats is still nascent.Here,we performed a degenerate primer screening and next-generation sequencing analysis of 112 bats,collected from Hainan Province,China.Three coronaviruses,namely bat betacoronavirus(Bat CoV)CD35,Bat CoV CD36 and bat alpha-coronavirus CD30 were identified.Bat CoV CD35 genome had 99.5%identity with Bat CoV CD36,both sharing the highest nucleotide identity with Bat Hp-betacoronavirus Zhejiang2013(71.4%),followed by SARS-CoV-2(54.0%).Phylogenetic analysis indicated that Bat CoV CD35 formed a distinct clade,and together with Bat Hp-betacoronavirus Zhejiang2013,was basal to the lineage of SARS-CoV-1 and SARS-CoV-2.Notably,Bat CoV CD35 harbored a canonical furin-like S1/S2 cleavage site that resembles the corresponding sites of SARS-CoV-2.The furin cleavage sites between CD35 and CD36 are identical.In addition,the receptor-binding domain of Bat CoV CD35 showed a highly similar structure to that of SARS-CoV-1 and SARS-CoV-2,especially in one binding loop.In conclusion,this study deepens our understanding of the diversity of coronaviruses and provides clues about the natural origin of the furin cleavage site of SARS-CoV-2.

关 键 词：BAT CORONAVIRUS SARS-CoV-2 Betacoronavirus FURIN Cleavage site 

分 类 号：S852.65[农业科学—基础兽医学]