Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport(ESCRT)protein  

作　　者：Wenyuan Shen Chang Liu Yue Hu Qian Ding Jiabin Feng Zhou Liu Xiaohong Kong 

机构地区：[1]Laboratory of Medical Molecular Virology,School of Medicine,Nankai University,Tianjin,300071,China [2]Department of Spine Surgery,The Second Hospital of Shandong University,Cheeloo College of Medicine,Shandong University,Jinan,250033,China [3]International Science and Technology Cooperation Base of Spinal Cord Injury,Tianjin Key Laboratory of Spine and Spinal Cord Injury,Tianjin Medical University General Hospital,Tianjin,300052,China [4]Department of Infectious Diseases,Tianjin Second People's Hospital,Tianjin,300192,China

出　　处：《Virologica Sinica》2023年第3期448-458,共11页中国病毒学（英文版）

基　　金：We greatly appreciate Prof.Charles Wood(University of Nebraska,Lincoln,USA)for the gift of the infectious molecular clones(pNL4.3,pNL4.3ΔEnvEGFP,and pVSV-G).;the National Natural Science Foundation of China(81571987);Natural Science Foundation of Tianjin Municipal Science and Technology Commission(20JCQNJC01750,21JCQNJC01600).

摘　　要：Human immunodeficiency virus-1(HIV-1)encodes simply 15 proteins and thus depends on multiple host cellular factors for virus reproduction.Spastin,a microtubule severing protein,is an identified HIV-1 dependency factor,but the mechanism regulating HIV-1 is unclear.Here,the study showed that knockdown of spastin inhibited the production of the intracellular HIV-1 Gag protein and new virions through enhancing Gag lysosomal degradation.Further investigation showed that increased sodium tolerance 1(IST1),the subunit of endosomal sorting complex required for transport(ESCRT),could interact with the MIT domain of spastin to regulate the intracellular Gag production.In summary,spastin is required for HIV-1 replication,while spastin-IST1 interaction facilitates virus production by regulating HIV-1 Gag intracellular trafficking and degradation.Spastin may serve as new target for HIV-1 prophylactic and therapy.

关 键 词：HIV-1 replication SPASTIN Gag production Lysosomal degradation Endosomal sorting complex required for transport(ESCRT) 

分 类 号：S941.41[农业科学—水产养殖]