RNA-Seq技术在珍稀濒危植物研究中的应用进展  

Application Progress of RNA-Seq Technology in Rare and Endangered Plants

作　　者：倪馨宇 贺俊英[1,2] 燕孟娇 杜超 NI Xinyu;HE Junying;YAN Mengjiao;DU Chao(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022;Key Laboratory of Biodiversity conservation and Sustainable utilization for College and University of Inner Mongolia Autonomous Region,Hohhot 010022;Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031)

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022 [2]内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室,呼和浩特010022 [3]内蒙古自治区农牧业科学院植物保护研究所,呼和浩特010031

出　　处：《植物研究》2023年第4期481-492,共12页Bulletin of Botanical Research

基　　金：内蒙古自治区自然科学基金项目(2019LH03005);内蒙古师范大学引进高层次人才科研启动经费项目(2018YJRC014)。

摘　　要：珍稀濒危植物是自然界当中的重要植物资源,对研究植物系统进化、生态环境恢复、植物抗逆生理、挖掘优异抗逆基因等方面具有重要意义,但大部分珍稀濒危植物的遗传信息缺乏,严重制约了其保护和利用工作的开展。珍稀濒危植物的基因组较大、基因信息复杂、遗传背景不清晰,因此对其进行基因组测序相对困难,而RNA-Seq技术拥有可以对无参基因组物种直接进行测序的优势,近年来在珍稀濒危植物的研究中得到青睐。文章简介了RNA-Seq技术,综合近年研究总结了RNA-Seq技术在珍稀濒危植物抗逆机制、次生代谢、生长发育调控及分子标记开发4个主要应用方向的研究进展。此外,还对RNA-Seq技术在珍稀濒危植物研究中的应用前景进行了展望,同时在此基础上提出珍稀濒危植物转录组新研究思路的可能性。Rare and endangered plants are important plant resources in nature,and play a key role in the study of plant phylogeny,ecosystem restoration,plant stress resistance physiology,and the excavation of stressresistance genes.However,the lack of genetic information has severely restricted the conservation and utilization in most endangered plant.Due to the large genomes,complex genetic information,and unclear genetic backgrounds of endangered plants,it is relatively difficult to sequence the genomes of endangered plants.In recent years,RNA-Sequencing could directly sequence non-reference genomic species,what makes it to be widely used in the study of endangeredplants.In addition,this paper presented the prospect of the application of RNA-Sequencing technology in the study of endangered plants and suggested the new ideas of transcriptome using in the endangered plants in future.

关 键 词：转录组测序技术 珍稀濒危植物 抗逆机制 次生代谢 

分 类 号：Q94[生物学—植物学]