巨桉EgrWAT1基因克隆和功能初步分析  

Cloning and Functional Analysis of EgrWAT1 Gene in Eucalyptus grandis

作　　者：张昊楠 陈珊珊 徐建民[2] 罗萍 王晓萍 许志茹[1] 范春节[2,3] ZHANG Haonan;CHEN Shanshan;XU Jianmin;LUO Ping;WANG Xiaoping;XU Zhiru;FAN Chunjie(School of Life Science,Northeast Forestry University,Harbin 150040;Laboratory of State Forestry and Grassland Administration,Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou 510520;State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University/Chinese Academy of Forestry,Harbin/Beijing 150040/100091)

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]国家林业和草原局热带林木培育重点实验室,中国林业科学研究院热带林业研究所,广州510520 [3]林木遗传育种国家重点实验室,东北林业大学/中国林业科学研究院,哈尔滨/北京150040/100091

出　　处：《植物研究》2023年第4期601-611,共11页Bulletin of Botanical Research

基　　金：中国林业科学研究院基本科研业务费专项资金项目(CAFYBB2020ZB004)。

摘　　要：为探究WALLS ARE THIN(WAT1)在木本植物中木材形成以及响应胁迫中的作用,利用生物信息学工具进行分析,并以巨桉(Eucalyptus grandis)为材料克隆EgrWAT1S及其另一转录本EgrWAT1L,通过实时荧光定量PCR(qRT-PCR)探究其在不同组织、节间以及响应胁迫时的表达模式。结果表明:EgrWAT1S在韧皮部表达量较高,而EgrWAT1L主要表达在根部。在茉莉酸甲酯(MeJA)、水杨酸(SA)处理和盐胁迫以及缺磷、缺硼处理时,其表达存在着明显不同的模式,在MeJA、SA处理时甚至存在着相反的表达模式。这些结果表明EgrWAT1L基因可能通过转录调控来影响EgrWAT1S表达和进一步的蛋白翻译来响应激素和胁迫处理。为进一步研究WAT1基因在巨桉生长发育过程中的作用和调控方式提供基础,也为将来桉树的分子育种提供可能。In order to explore the role of WALLS ARE THIN(WAT1)in wood formation and response to stress in woody plants,bioinformatics tools was used for analysis,and quantitative Real-time PCR(qRT-PCR)was used to investigate the expression patterns of EgrWAT1L and EgrWAT1S in different tissues,internodes and in response to different stresses,and the gene EgrWAT1S and its other transcript EgrWAT1L were cloned from Eucalyptus grandis.The results showed that the EgrWAT1S was highly expressed in phloem,while EgrWAT1L was mainly expressed in roots,and the expression patterns were significantly different under methyl jasmonate(MeJA)and salicylic acid(SA)treatment,salt stress,phosphorus(P)and boron(B)deficiency,and even opposite under MeJA and SA.These results suggested that EgrWAT1L might affect EgrWAT1S expression through transcriptional regulation and further protein translation in response to hormone and stress treatments.The studies provided a basis for further elucidate the function in the growth and development of EgrWAT1 gene and also provided a possibility for future molecular breeding of Eucalyptus.

关 键 词：巨桉 WAT1 可变剪切 基因克隆 表达模式 

分 类 号：S792.39[农业科学—林木遗传育种]