超声辅助酶解提取五味子多糖及其抗细胞氧化应激研究  

Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Schisandra chinensis and the Effects on Anti-Oxidative Stress in Cells

作　　者：朱翰林 赵恒 翟博文 张卯玉 付玉杰 ZHU Hanlin;ZHAO Heng;ZHAI Bowen;ZHANG Maoyu;FU Yujie(Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040;College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040;College of Forestry,Beijing Forestry University,Beijing 100083)

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]东北林业大学化学化工与资源利用学院,哈尔滨150040 [3]北京林业大学林学院,北京100083

出　　处：《植物研究》2023年第4期631-640,共10页Bulletin of Botanical Research

基　　金：高等学校学科创新引智计划项目(B20088);黑龙江头雁创新团队计划(林木遗传育种创新团队)。

摘　　要：以五味子(Schisandra chinensis)干果为原料,采用超声辅助纤维素酶酶解法提取五味子多糖,研究五味子多糖的超声辅助酶解工艺与抗细胞氧化应激活性。以多糖得率为指标,采用单因素试验和Box-Behnken响应面试验研究加酶量、超声功率、提取时间和料液比对五味子多糖得率的影响,优化得到五味子多糖最佳超声辅助酶解提取工艺条件;建立脂多糖(LPS)诱导的HepG2细胞氧化应激损伤模型,以丙二醛(MDA)、超氧化物歧化酶(SOD)和活性氧(ROS)水平为指征,研究五味子多糖的抗细胞氧化应激能力。结果表明:五味子多糖的最佳提取工艺条件为加酶量1420 U·g^(-1),超声功率500 W,提取时间46 min,料液比1 g:32 mL,该条件下五味子多糖得率为22.25%;此外,所提取得到的五味子多糖可提高LPS诱导HepG2细胞中的SOD水平,并可降低细胞中MDA的积累量和ROS荧光强度,有效缓解了LPS诱导的HepG2细胞氧化应激反应。优化确立了超声辅助纤维素酶提取五味子多糖最佳工艺条件,发现所提取得到的五味子多糖有良好抗氧化应激能力。The polysaccharides of Schisandra chinensis were extracted by ultrasonic-assisted cellulase enzymolysis from the dried fruit of S.chinensis as raw material,and the extraction process and anti-oxidative stress activity of S.chinensis polysaccharides were studied.Using polysaccharide yield as index,single factor experiment and Box-Behnken response surface test were used to investigate the effects of enzyme concentration,ultrasonic power,extraction time and solid-liquid ratio on polysaccharide yield of S.chinensis,and the ultrasonic-assisted enzymatic extraction process was optimized.A lipopolysaccharide(LPS)induced oxidative stress damage model of HepG2 cells was established,and the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and reactive oxygen species(ROS)were used as indicators,and the anti-oxidative stress ability of S.chinensis polysaccharides was investigated.The results showed that the optimal extraction conditions as followed:the enzyme concentration was 1420 U·g^(-1),the ultrasonic power was 500 W,the extraction time was 46 min,the solid-liquid ratio was 1 g∶32 mL.Under these conditions,the yield of polysaccharides was 22.25%.Moreover,the extracted S.chinensis polysaccharides might improve the SOD level in the LPS-induced HepG2 cells,but reduce the MDA accumulation and ROS fluorescence intensity in the cells,and effectively relieve the LPS-induced oxidative stress in HepG2 cells.The extraction conditions of polysaccharides from S.chinensis were optimized,and it was found that the extracted polysaccharides had good antioxidant stress ability.

关 键 词：五味子 多糖 响应面法优化 氧化应激 

分 类 号：Q539[生物学—生物化学]