基于非靶向代谢组学技术探究喂养方式对剖宫产婴儿粪便中代谢物的影响  

The effect of feeding methods on fecal metabolites of infants delivered by cesarean section based on non-targeted metabolomics

作　　者：鲁曦 赵馨[1] 张明新 李国花 周宁宁 卫飞鹏 赵燕妮 Lu Xi;Zhao Xin;Zhang Mingxin;Li Guohua;Zhou Ningning;Wei Feipeng;Zhao Yanni(School of Food Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China;Department of Interventional Pain,Tangdu Hospital,Air Force Medical University,Xi′an 710032,China)

机构地区：[1]陕西科技大学食品科学与工程学院,西安710021 [2]空军军医大学唐都医院介入疼痛科,西安710032

出　　处：《中华实用儿科临床杂志》2023年第7期515-520,共6页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(31970115);陕西省重点研发计划(2022NY-030)。

摘　　要：目的探讨母乳和混合喂养对剖宫产婴儿粪便代谢物的影响。方法横断面研究。2021年秋季与2022年冬季分别在西安市南、北两家母婴护理机构收集了23例1月龄健康剖宫产婴儿粪便样本, 将样品分为母乳喂养组(11例)和混合喂养组(12例)。基于非靶向代谢组学方法, 借助气相色谱-质谱联用技术, 探索不同喂养方式下剖宫产婴儿粪便代谢产物的变化情况, 利用非参数Mann-Whitney U检验方法筛选差异代谢物, 并对差异代谢产物进行代谢通路分析。结果共定性出155种代谢物, 包括糖及糖类衍生物57种, 脂肪酸34种, 有机酸25种, 氨基酸22种, 酯类8种, 核苷4种, 维生素3种以及2种其他物质。通过对差异代谢物的相对含量进行分析, 发现母乳喂养组中部分糖及糖的衍生物含量较高, 而混合喂养组中氨基酸、有机酸及脂肪酸含量较高。通过对差异代谢物的代谢通路进行分析, 共获得28条代谢通路, 其中丙氨酸、天冬氨酸和谷氨酸代谢, 缬氨酸、亮氨酸和异亮氨酸代谢, 精氨酸代谢及柠檬酸循环(TCA循环)等代谢通路对婴儿健康有一定的影响。结论喂养方式对剖宫产婴儿粪便中的代谢物存在影响, 且混合喂养可能在一定程度上促进剖宫产婴儿肠道中TCA循环和氨基酸代谢的过程。Objective To explore the effect of breast feeding versus mixed feeding on fecal metabolites of infants delivered by cesarean section.Methods This was a cross-sectional study.Fecal samples were collected from 23 healthy 1-month-old infants delivered by cesarean section from autumn 2021 and winter 2022 in two maternal and infant care facilities in the North and South of Xi′an city.The samples were divided into the breast feeding group(11 cases)and mixed feeding group(12 cases).Fecal metabolites were analyzed by the non-targeted metabolomic approach and gas chromatography-mass spectrometry coupling,and differentially expressed fecal metabolites between groups were screened using the non-parametric Mann-Whitney U test.Metabolic pathways enriched in them were further analyzed.Results A total of 155 metabolites were characterized,including 57 sugars and sugar derivatives,34 fatty acids,25 organic acids,22 amino acids,8 esters,4 nucleosides,3 vitamins and 2 other substances.The relative contents of the differentially expressed fecal metabolites were measured,and it was found that some types of sugars and sugar derivatives were highly expressed in the fecal samples of breast feeding group,while amino acids,organic acids and fatty acids were highly expressed in those of the mixed feeding group.A total of 28 metabolic pathways enriched in differentially expressed fecal metabolites were obtained.Among them,alanine,aspartic acid and glutamic acid metabolism,valine,leucine and isoleucine metabolism,arginine metabolism and the tricarboxylic acid(TCA)cycle influenced infant health.Conclusions Feeding methods have an effect on the fecal metabolites in infants delivered by cesarean section born infants,and mixed feeding may speed up the process of TCA cycle and amino acid metabolism in the intestine of infants delivered by cesarean section to a certain extent.

关 键 词：非靶向代谢组学 喂养方式 剖宫产婴儿 粪便 差异代谢物 

分 类 号：R174[医药卫生—妇幼卫生保健]