促生长植物激素对桑树叶片衰老过程生理生化指标的影响  

Effects of growth promoting hormones on physiological and biochemical indexes of mulberry leaves during senescence

作　　者：朱燕[1] 魏佳[1] 许自龙 林天宝[1] 杨升[2] 刘岩[1] 吕志强[1] 刘培刚[1] ZHU Yan;WEI Jia;XU Zilong;LIN Tianbao;YANG Sheng;LIU Yan;LYU Zhiqiang;LIU Peigang(Institute of Sericulture and Tea,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Institute of Subtropical Crops,Zhejiang Academy of Agricultural Sciences,Wenzhou 325005,Zhejiang,China)

机构地区：[1]浙江省农业科学院蚕桑与茶叶研究所,浙江杭州310021 [2]浙江省农业科学院亚热带作物研究所,浙江温州325005

出　　处：《浙江农业学报》2023年第6期1278-1285,共8页Acta Agriculturae Zhejiangensis

基　　金：浙江省公益技术研究计划(LGN21C160013);“十四五”农业新品种选育重大育种专项蚕桑协作项目(2021C02072-5);现代农业产业技术体系建设专项(CARS-18-270203);温州市基础性农业科技项目(N2020004)。

摘　　要：为研究促生长类激素对于桑树叶片衰老过程的影响,以强桑1号(迟衰)和荷叶白(早衰)为研究对象,测定品种间生长素(auxin,IAA)、细胞分裂素(cytokinin,CTK)、赤霉素(gibberellin,GA)含量差异,以及外源激素与抑制剂处理后内源激素含量及抗氧化酶等主要生理生化指标变化情况。结果表明:强桑1号叶片中IAA、CTK和GA含量显著高于同时间节点的荷叶白;同时发现经外源激素处理后3者含量显著增加(P<0.05),抑制剂处理后含量显著(P<0.05)降低。另外,经外源激素处理后,2个品种叶片中过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)及超氧化物歧化酶(superoxide dismutase,SOD)活性显著提升,可溶性蛋白(soluble protein,SP)和脯氨酸(proline,Pro)含量显著提升,而丙二醛(malondialdehyde,MDA)含量显著降低(P<0.05);经抑制剂处理后,桑树叶片中的CAT、POD、SOD活性,及MDA、SP、Pro含量变化因抑制剂种类和桑树品种不同而存在差异。The content of auxin(IAA),cytokinin(CTK),and gibberellin(GA)and main physiological and biochemical indexes in leaves of Qiangsang 1(late senescence)and Heyebai(early senescence)before and after application of exogenous hormones and inhibitors were determined in this study for examining the effect of growth-promoting hormones on the senescence of mulberry leaves.The results showed that the IAA,CTK and GA in Qiangsang 1 were significantly higher than those of Heyebai at the same time;the content of them were up-regulated significantly after treatment by exogenous hormones(P<0.05)and down-regulated significantly after treatment by those inhibitor(P<0.05).In addition,after treatment by exogenous hormones,the activities of CAT,POD,and SOD,and SP content and Pro content in leaves were up-regulated significantly,while MDA content was down-regulated significantly(P<0.05).The activities of CAT,POD and SOD,MDA content,SP content and Pro content in mulberry leaves changed differently due to the mulberry varieties and the types of inhibitors after treatment with inhibitors.

关 键 词：促生长激素 桑树 叶片 衰老 

分 类 号：S888.2[农业科学—特种经济动物饲养]