三种伞形科蔬菜作物棕榈酰基转移酶基因家族的鉴定与分析  

Identification and analysis of PAT gene family in three kinds of Apiaceae vegetable crops

作　　者：张新业 李文静 朱姝 孙艳香[1] 王聪艳[1] 闫训友[1] 周志国[1] ZHANG Xinye;LI Wenjing;ZHU Shu;SUN Yanxiang;WANG Congyan;YAN Xunyou;ZHOU Zhiguo(College of Life Science,Langfang Normal University,Langfang 065000,Hebei,China;Hebei Key Laboratory of Animal Diversity,Langfang 065000,Hebei,China;Langfang Key Laboratory of Cell Engineering and Application,Langfang 065000,Hebei,China)

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]河北省动物多样性重点实验室,河北廊坊065000 [3]廊坊市细胞工程与应用研究重点实验室,河北廊坊065000

出　　处：《浙江农业学报》2023年第6期1315-1327,共13页Acta Agriculturae Zhejiangensis

基　　金：河北省自然科学基金(C2020408006);国家重点研发计划(2019YFD1000305-09);河北省高等学校科学技术研究项目(BJ2018044)。

摘　　要：在全基因组水平鉴定胡萝卜(Daucus carota)、芹菜(Apium graveolens)、香菜(Coriandrum sativum)3种伞形科蔬菜作物的棕榈酰基转移酶(protein S-acyltransferase,PAT),对其家族成员进行生物信息学和表达分析,为深入探究该类蛋白在3种伞形科作物生长发育及抵御逆境胁迫中的作用提供参考。利用Basic Local Alignment Search Tool(BLAST)和hmmsearch程序,从胡萝卜、芹菜、香菜基因组中筛选、鉴定PAT家族成员;利用TBtools绘制PAT基因染色体分布图;利用Expasy分析PAT蛋白的等电点、分子量;利用TMHMM-2.0预测PAT蛋白跨膜区;利用CELLO v.2.5、WoLF PSORT预测PAT蛋白的亚细胞定位情况;利用MEGA6构建系统发育进化树;利用MEME分析PAT蛋白保守基序;基于荧光定量PCR实验结果及转录组数据,利用TBtools绘制基因表达热图,分析PAT基因的组织表达特性及胁迫响应情况。结果表明,在胡萝卜、芹菜及香菜基因组中各存在27、25、27个PAT基因,分别命名为DcPAT 1~27、AgPAT 1~25、CsPAT 1~27。这些PAT成员不均匀地分布于染色体上,理化性质存在差异,且均为跨膜蛋白。系统进化分析将伞形科及来源于其他物种的PAT蛋白分为7个类群(Group1~7),伞形科作物PAT蛋白在7个类群中均有分布。结构分析表明,所有的PAT蛋白均含有DHHC-CRD结构域,该序列内的保守氨基酸残基在不同物种间已发生变异。荧光定量PCR和转录组数据分析表明,PAT基因在胡萝卜、芹菜、香菜不同组织间的表达水平有差异,但有部分成员在不同组织间均呈现高水平或低水平表达,另有部分DcPAT基因在盐胁迫和低温胁迫处理后,表达水平发生改变。The objective of this study was to identify the PAT gene family in three kinds of Apiaceae vegetable crops(Daucus carota,Apium graveolens,Coriandrum sativum)at genome scale and provide a reference for further exploration of their roles in plant growth,development and stress tolerance.The PAT proteins were identified from genomes of the three species by BLAST and hmmsearch.TBtools was used to depict chromosome locations of PAT genes.The isoelectric point and molecular weight were analyzed by Expasy.TMHMM-2.0,CELLO v.2.5 and WoLF PSORT were used to predict transmembrane regions and subcellular localization of PAT.MEGA6 was used to construct the phylogenetic tree,and MEME was used to analyze conserved motifs of PAT proteins.Based on real time quantitative PCR results and transcriptome data,TBtools was used to draw gene expression heat map,and tissue expression characteristics and responses to stress of PAT genes were analyzed.The results showed that 27,25,27 PAT gene family members were identified in Daucus carota,Apium graveolens,Coriandrum sativum respectively,which were named as DcPAT1-27,AgPAT1-25,CsPAT 1-27.These members with transmembrane regions distributed unevenly on chromosomes of three species,and they had different physical and chemical properties.According to phylogenetic analysis,PAT proteins from Apiaceae and other species were divided into 7 groups,and Apiaceae PAT proteins were distributed throughout all groups.Structural analysis showed that all PAT proteins had DHHC-CRD domain,and conserved amino acid residues in this domain had varied among different species.Gene expression analysis showed that the expression levels of PAT genes were different across different tissues of Daucus carota,Apium graveolens,Coriandrum sativum.Some members showed high or low expression levels in all tissues,and the expression levels of some DcPAT genes had changed after salt stress or low temperature stress.

关 键 词：伞形科 棕榈酰基转移酶 基因家族 生物信息学分析 表达分析 

分 类 号：S63[农业科学—蔬菜学]