侵染青海辣椒的辣椒隐症病毒2基因组克隆与分析  

Cloning and identification of Pepper cryptic virus 2 in Qinghai Province,China

作　　者：罗海林 袁雷 闫佳会 郭青云 LUO Hailin;YUAN Lei;YAN Jiahui;GUO Qingyun(Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province,Scientific Observing and Experimental Station of Crop Pest in Xining,Ministry of Agriculture and Rural Affairs,Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农林科学院,青海省农业有害生物综合治理重点实验室,农业农村部西宁作物有害生物科学观测实验站,青海西宁810016

出　　处：《浙江农业学报》2023年第6期1368-1374,共7页Acta Agriculturae Zhejiangensis

基　　金：青海省农业有害生物综合治理重点实验室(2022-ZJ-Y10);青海省国际科技合作项目(2022-HZ-806)。

摘　　要：辣椒隐症病毒2(Pepper cryptic virus 2,PCV2)是近年来发现的辣椒新病毒之一,常与其他病毒复合侵染,影响辣椒的产量和品质。2021年从青海海东市采集疑似病样叶片,采用RT-PCR和基因克隆等方法,获得PCV2青海分离物的两条dsRNA,序列全长分别为1579、1435 bp。核苷酸相似性比对发现,PCV2青海分离物两条RNA序列与已报道的其他地区PCV2分离物的序列相似性高于95%,系统进化分析结果显示,PCV2青海分离物的两条RNA均与PCV2分离物Chiltepin 53处于独立的分支,结果表明,青海辣椒上检测得到的病毒为辣椒隐症病毒2。将其与PCV2-QH和PCV2-XJ的核苷酸序列进行分析,结果显示,二者存在多位点差异。本研究首次报道PCV2在青海发生。Pepper cryptic virus 2 is one of the new viruses in pepper.It often infects with other viruses,seriously reducing the yield and quality of pepper.In 2021,suspected pepper s leaf samples were collected from Haidong,Qinghai.Two dsRNA complete gene sequences of PCV2 were obtained by using RT-PCR and gene cloning.The two-length sequence of Qinghai isolates were 1579 bp and 1435 bp.The nucleotide sequence identities against other Pepper cryptic virus 2 isolates were over 95%.Phylogenetic analysis showed that PCV2 Qinghai isolate clustered with Chiltepin53 in a sub-clade.All these results showed that the virus infected pepper was an isolate of PCV2.Analyzing the nucleotide sequence of PCV2-QH and PCV2-XJ,the results showed that there were multiple differences.This is the first report of PCV2 infecting pepper in Qinghai.

关 键 词：辣椒 辣椒隐症病毒2(PCV2) 全基因组 序列分析 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]