基于核转录因子-κB信号通路探讨铁包金巴布膏治疗腰椎间盘突出症的作用机制  

To study the intervention mechanism of Tiebaojin Cataplasm based on NF-κB signaling pathway

作　　者：安娟 李振宇 常裕绅 张子鸣 叶子丰 匡浩铭 匡建军[2] 戎宽[3] AN Juan;LI Zhenyu;CHANG Yushen;ZHANG Ziming;YE Zifeng;KUANG Haoming;KUANG Jianjun;RONG Kuan(Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学中医药研究院,长沙410208 [2]湖南省中医药研究院中医临床研究所 [3]湖南省中医药研究院附属医院骨伤科

出　　处：《环球中医药》2023年第6期1091-1096,共6页Global Traditional Chinese Medicine

基　　金：湖南省卫生健康委科研计划(202204074273);湖南创新型省份建设专项(2022JJ60076);长沙市科技计划(KH2201063);国家中医药管理局全国名老中医药专家传承工作室建设项目(国中医药人教发[2016]42号);湖南中医药大学研究生科研创新项目(2022CX191)。

摘　　要：目的基于核转录因子-κB(nuclear factorκB,NF-κB)信号通路探讨铁包金巴布膏治疗腰椎间盘突出症的作用机制。方法SPF级3月龄雄性大鼠40只中随机取10只设为正常组,其余的大鼠用自体髓核移植法建立腰椎间盘突出症(lumber disc herniation,LDH)大鼠模型,造模成功后随机分为模型组、铁包金巴布膏组、阳性药物组,每组10只。正常组及模型组以适量生理盐水涂抹L5椎旁,每日1次,连续30天。铁包金巴布膏组贴敷1.54 cm×2.09 cm铁包金巴布膏,每日1次,连续30天。阳性药物组以扶他林软膏外涂,每日1次,连续30天。给药30天后,禁食12小时,麻醉大鼠,腹主动脉采血,并暴露右侧L5神经根,冷冻台上取受压神经根后处死大鼠。检测大鼠的神经功能评分;苏木精-伊红(hematoxylin-eosin,HE)染色法观察大鼠背根神经节组织形态学变化;酶联免疫吸附测定法(enzyme linked immunosorbent assay,ELISA)测量大鼠血清白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)含量;实时荧光定量聚合酶链式反应(real-time quantitative PCR,qRT-PCR)检测髓样细胞分化因子88(myeloid differentiation factor88,MyD88)、NF-κB p65的mRNA表达水平。结果(1)神经功能评分结果显示,与模型组相比,在干预第10天、20天和30天,铁包金巴布膏组、阳性药物组的评分均下降(P<0.05);(2)HE染色结果显示,与模型组相比,铁包金巴布膏组、阳性药物组大鼠背根神经节的结构形态较规则,细胞核较均匀,胞浆空泡状数量较少,尼氏小体排列均匀;(3)ELISA检测结果显示,与正常组相比,模型组的大鼠血清中炎性因子IL-1β、TNF-α含量水平显著升高(P<0.05),与模型组相比,铁包金巴布膏组、阳性药物组的IL-1β、TNF-α含量水平显著降低(P<0.05);(4)qRT-PCR结果显示,与正常组相比,模型组、铁包金巴布膏组、阳性药物组NF-κB p65及MyD88 mRNA的表达水平均升高(P<0.05);与模型组�Objective Explore the mechanism of Tiebaojin cataplasm in the treatment of lumbar disc herniation based on the signal pathway of nuclear factorκB(NF-κB).Methods Ten of 40 SPF 3-month-old male rats were randomly selected as the normal group.The other were used to establish the LDH rat model by Autologous nucleus pulposus transplantation.After successful modeling,the rats were randomly divided into model group,Tiebaojin cataplasm group,positive drug group,10 rats in each group.The normal group and the model group were smeared L5 paravertebral with normal saline,once a day for 30 days.The rats in the Tiebaojin cataplasm group were treated with 1.54 cm×2.09 cm plaster,once a day for 30 days.The positive drug group was treated with voltaren ointment,once a day for 30 days.After 30 days,fasting for 12 hours,anesthetized rats,abdominal aorta blood collection.The right L5 nerve root was exposed,and the compressed nerve root was harvested from the cryopreserved platform and the rats were killed.The nerve function score of rats were determined.The histomorphological changes of dorsal root ganglion(DRG)were detected by hematoxylin eosin(HE)staining.The levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of MYD88 and NF-κB p65 were detected by real-time quantitative polymerase chain reaction(real-time PCR).Results(1)The results of nerve function score showed that compared with the model group,the scores of the Tiebaojin cataplasm group and the positive drug group were decreased at the 10th,20th and 30th day after intervention(P<0.05).(2)According to HE staining,compared with model group,the edge and nucleolus of nucleus were clearer in the Tiebaojin cataplasm group and the positive drug group.Nissl bodies in cytoplasm were arranged more evenly,and vacuoles in cytoplasm were reduced in the Tiebaojin cataplasm group and the positive drug group,and the positive drug group showed more significant improvement.(3)As revealed by ELISA res

关 键 词：腰椎间盘突出症 铁包金巴布膏 核转录因子-ΚB 炎症因子 髓样分化因子88 

分 类 号：R285.5[医药卫生—中药学]