重离子束辐射诱导细胞双链断裂(DSBs)损伤及修复机理的研究进展  

Progress of research on double-strand break damage induced by heavy-ion beam radiation and repair mechanism

作　　者：任军乐 郭晓鹏 雷彩荣 张苗苗[1,2,4] 柴冉 陆栋 REN Junle;GUO Xiaopeng;LEI Cairong;ZHANG Miaomiao;CHAI Ran;LU Dong(Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China;University of Chinese Academy of Sciences,Beijing 100049,China;Lanzhou University of Technology,Lanzhou 730050,China;Key Laboratory of Microbial Resources Development and Utilization of Gansu Province,Lanzhou 730070,China)

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院大学,北京100049 [3]兰州理工大学,兰州730050 [4]甘肃省微生物资源开发利用重点实验室,兰州730070

出　　处：《辐射研究与辐射工艺学报》2023年第3期1-10,共10页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(11905265和11975284);甘肃省自然科学基金项目(20JR5RA552);中国科学院“西部青年学者”(E023211Y)资助。

摘　　要：重离子束辐射能引发细胞DNA双链断裂,被认为是构成基因组不稳定因素之一。现有研究表明:同源末端连接、同源重组、单链退火和选择性末端连接在修复DNA双链断裂方面发挥着重要的作用,但是影响DNA双链断裂修复途径选择的因素目前仍不清楚。本文对近年重离子辐射细胞产生的DNA损伤特征和修复途径方面的新发现进行了综述,并从类型和分布、染色质状态、DNA末端结构、DNA末端切除、细胞周期方面解释了细胞DNA双链断裂修复途径的选择机制。这对细胞DNA损伤修复的研究具有重要意义,为重离子辐射技术在生物学效应研究方面提供了参考。Heavy-ion beam radiation can cause cell DNA double-strand breaks(DSBs),which are factors that lead to genomic instability.Existing studies have demonstrated that homologous end joining,homologous recombination,single-strand annealing,and selective end joining play critical roles in the repair of DNA DSBs.However,the factors that affect the selection of repair pathways for DNA DSBs remain unclear.In this study,recent findings on DNA damage characteristics and repair pathways generated by heavy-ion radiation cells are reviewed,and the selection mechanism of DSB repair pathways in cells is explained in terms of the types and distribution of DNA DSBs,chromatin status,DNA terminal structures,DNA terminal excision,and cell cycles.This review is of great significance for the study of DNA damage repair and provides a reference for investigating the biological effects of heavy-ion radiation technology.

关 键 词：重离子束辐射 DNA损伤 簇状DSBs DNA损伤修复 

分 类 号：Q691[生物学—生物物理学] TL99[核科学技术—核技术及应用]