基于F2和RIL群体鉴定棉花抗黄萎病主效QTL  

Identification of major QTL for Verticillium wilt resistance in cotton by using F2 and RIL population

作　　者：赵云雷[1] 王文菊 陈伟[1] 王海红 朱喜霞 杨继华 鲁宁宁[1] 赵佩[1] 桑晓慧[1] 崔艳利 敦磊 王红梅[1] Zhao Yunlei;Wang Wenju;Chen Wei;Wang Haihong;Zhu Xixia;Yang Jihua;Lu Ningning;Zhao Pei;Sang Xiaohui;Cui Yanli;Dun Lei;Wang Hongmei(Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology/National Engineering Research Center of Cotton Biology Breeding and Industrial Technology,Anyang,Henan 455000,China;Agricultural and Rural Bureau of Yindu District,Anyang,Henan 455000,China;Shandong Zhongli Cotton Technology Company Limited,Dongying,Shandong 257500,China)

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室/棉花生物育种及产业技术国家工程研究中心,河南安阳455000 [2]安阳市殷都区农业农村局,河南安阳455000 [3]山东众力棉业科技有限公司,山东东营257500

出　　处：《棉花学报》2023年第2期101-116,共16页Cotton Science

基　　金：国家自然科学基金(32172082);中央级公益性科研院所基本科研业务费专项(1610162022004,1610162022036)。

摘　　要：【目的】通过对棉花抗黄萎病性状进行数量性状位点(quantitative trait locus,QTL)定位,鉴定可以应用于育种实践的能稳定检测到的主效QTL,为棉花抗黄萎病遗传改良奠定分子基础。【方法】以抗黄萎病品种中植棉2号和感黄萎病品种冀棉11号为亲本杂交的F2群体和重组自交系(recombinant inbred lines,RIL)群体作为作图群体,在对2个群体进行多环境黄萎病抗性鉴定和简单重复序列(simple sequence repeat,SSR)分子标记检测的基础上进行遗传连锁图谱构建,利用完备区间作图法进行QTL定位,并对获得的主效QTL置信区间进行候选基因挖掘。【结果】在F2:3家系和RIL群体中共检测到7个抗黄萎病QTL,能够在多个环境条件下重复检测到的QTL有4个,包括q VW-D05-1、qVW-D05-2、q VW-D05-4和qVW-D05-5。共线性分析表明上述4个QTL集中分布于D05染色体上2293776~3205058 bp和62407897~62582344 bp 2个区域。4个抗性QTL的聚合可以显著降低棉花黄萎病病情指数。qVW-D05-1在多个环境中的表型变异解释率均在10%以上,为抗黄萎病主效QTL。对qVW-D05-1置信区间内的基因进行了功能注释、黄萎病菌诱导后的表达模式分析和单核苷酸多态性(single nucleotide polymorphism,SNP)变异分析,初步推测Ghir_D05G038990、Ghir_D05G039060、Ghir_D05G039100、Ghir_D05G039110和Ghir_D05G039130为可能控制棉花黄萎病抗性的候选基因。【结论】在不同环境中共检测到7个抗黄萎病QTL,其中4个QTL被重复检测到,在主效QTL q VW-D05-1区间内筛选获得5个棉花抗黄萎病候选基因。这些稳定的抗黄萎病相关的QTL及其候选基因可应用于棉花抗黄萎病性状的分子标记辅助选择育种。[Objective]Conducting quantitative trait locus(QTL)mapping and detecting major QTL for Verticillium wilt resistance in cotton would lay the molecular foundation for the improvement of cotton disease resistance.[Method]In this study,the F2 population and the recombinant inbred line(RIL)population derived from the crossing between the Verticillium wilt resistant variety Zhongzhimian 2 and the Verticillium wilt susceptible variety Jimian 11 were used as the mapping population.Genetic linkage map construction and QTL mapping were performed by identifying Verticillium wilt resistance of the above two populations in multiple environments and detecting genotypes of the populations using simple sequence repeat(SSR)markers.The complete interval mapping method was used for QTL mapping.And the candidate resistance genes were screened from the major QTL intervals.[Result]Seven QTL for Verticillium wilt resistance were detected from the two populations.Of the seven QTL,four were detected in multiple environments,i.e.,qVW-D05-1,qVW-D05-2,qVW-D05-4,and qVW-D05-5.By analyzing the collinearity between the linkage map and physical map,the above four stable QTL were intensively located in two regions on D05 chromosome,and one region was 2293776-3205058 bp,the other was 62407897-62582344 bp.Polymerization of the above four resistant QTL could significantly decrease the disease index of cotton.qVW-D05-1 was identified as the major QTL,which can explain more than 10%phenotypic variation in multiple environments.The function annotation,expression pattern post Verticillium dahliae infection,and variation of single nucleotide polymorphism(SNP)were estimated for those genes located in the confidence interval of qVW-D05-1,and five genes,i.e.,Ghir_D05G038990,Ghir_D05G039060,Ghir_D05G039100,Ghir_D05G039110 and Ghir_D05G039130,were screened out to be candidate genes related to Verticillium wilt resistance in cotton.[Conclusion]Seven QTL for Verticillium wilt resistance were detected in different environments,among which four were identified a

关 键 词：棉花 黄萎病 QTL定位 重组自交系 基因挖掘 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]