5种牧草盲蝽寄主植物挥发物鉴别、选择反应及油菜诱集带在棉田的诱集效果  

Electrophysiological and behavioral responses of Lygus pratensis to plant volatiles and regulation of Brassica campestris trapping belt on L.pratensis in cotton fields

作　　者：夏欣 郑艺翔 姚成层 苟长青[1] 冯宏祖[1] Xia Xin;Zheng Yixiang;Yao Chengceng;Gou Changqing;Feng Hongzu(Tarim University/Key Laboratory of Xinjiang Production and Construction Corps in Comprehensive Agricultural Pest Management in Southern Xinjiang/Scientific Observing and Experimental Station of Crop Pests in Aral,Ministry of Agriculture and Rural Affairs,Aral,Xinjiang 843300,China)

机构地区：[1]塔里木大学/南疆农业有害生物综合治理兵团重点实验室/农业农村部阿拉尔作物有害生物科学观测实验站,新疆阿拉尔843300

出　　处：《棉花学报》2023年第2期128-137,共10页Cotton Science

基　　金：国家自然科学基金(32272539)。

摘　　要：【目的】旨在明确寄主植物挥发物在牧草盲蝽成虫化学通讯中的作用,以及油菜作为诱集植物对棉田牧草盲蝽的诱集和控制效果,为该虫植物源引诱剂的研发和生态调控提供基础资料。【方法】采用固相微萃取技术和气-质联用技术分析鉴定寄主植物挥发物成分;利用触角电位(electroantennography,EAG)仪和四臂嗅觉仪测定牧草盲蝽成虫对所选挥发物的EAG反应和趋向行为反应,同时在棉田种植油菜诱集带,调查分析诱集带及其临近棉田和对照田(未种植油菜的棉田)牧草盲蝽的种群数量。【结果】在5种牧草盲蝽寄主植物中共鉴定出挥发物25种,其中油菜9种、灰绿藜9种、棉花11种、龙葵10种、马齿苋6种;牧草盲蝽雌虫对苯乙醛的EAG反应相对值最高,趋向行为反应中苯乙醛、异硫氰酸仲丁酯对牧草盲蝽雌虫的吸引力分别达到了极显著和显著水平;直接测定法试验证明油菜较其他寄主植物对牧草盲蝽更具有吸引力;棉田油菜诱集带对牧草盲蝽有很好的诱集作用,棉田牧草盲蝽的发生量明显减少。【结论】苯乙醛和异硫氰酸仲丁酯对牧草盲蝽雌虫的吸引力达到了极显著或显著水平,可以作为潜在的引诱剂候选组分;油菜诱集带对牧草盲蝽有很好的诱集效果,是一种较理想的牧草盲蝽诱集植物。[Objective]The aim of this study was to clarify the role of host plant volatiles in the chemical communication of Lygus pratensis adults,and the effects of rapeseed(Brassica campestris L.)as a trap plant on the trapping and control in cotton fields,so as to provide basic informations for the development and ecological regulation of plant-derived attractors for the insect.[Method]Solid phase microextraction(SPME)and gas-mass spectrometry(GC-MS)were used to analyze and identify the volatiles of host plants.The electroantennography(EAG)and four-arm olfactory instrument were used to measure the EAG response and tendency behavior response of L.pratensis adult to selected volatiles.At the same time,cotton fields were planted with rapeseed attractor zone,and the populations of L.pratensis in the attractor zone and its adjacent cotton fields and control fields(without rapeseed cultivation)were investigated and analyzed.[Result]A total of 25 volatile compounds were identified in 5 species of host plants of L.pratensis,including 9 compounds were identified in Brassica napus,9 in Chenopodium glaucum,11 in Gossypium hirsutum L.,10 in Solanum nigrum and 6 in Portulaca oleracea.The EAG response of female L.pratensis to phenylacetaldehyde was the highest,and the attraction of phenylacetaldehyde and S-butyl isothiocyanate to female L.pratensis reached an extremely significant and significant level,respectively.The direct assay showed that rapeseed was more attractive to L.pratensis than other host plants.The rapeseed trapping zone in cotton field had a good trapping effect on L.pratensis,and the occurrence of L.pratensis in cotton field was significantly reduced.[Conclusion]The attraction of phenylacetaldehyde and S-butyl isothiocyanate to the female L.pratensis reached an extremely significant or significant level,which could be used as potential attractant candidates.The rapeseed trapping belt planted in cotton field has a good trapping effect on L.pratensis,which is an ideal trapping plant for L.pratensis.

关 键 词：牧草盲蝽 植物挥发物 嗅觉行为反应 触角电位反应 油菜诱集带 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]