不同剂量rhBMP2修复兔桡骨骨缺损的比较  

Comparison of different doses of rhBMP2 used for repairing radial bone defect in rabbit

作　　者：魏彬 黄景辉 马腾 夏冰 李胜友 郝一鸣 郑毅 高雪 罗卓荆 WEI Bin;HUANG Jing-hui;MA Teng;XIA Bing;LI Sheng-you;HAO Yi-ming;ZHENG Yi;GAO Xue;LUO Zhuo-jing(Department of Orthopedics,Xijing Hospital,Air Force Military Medical University,Xi'an 710000,China;Hubei Hospital of Armed Police Force,Wuhan 430000,China)

机构地区：[1]空军军医大学西京医院骨科,陕西西安710000 [2]武警湖北省总队医院,湖北武汉430000

出　　处：《中国矫形外科杂志》2023年第11期1018-1023,共6页Orthopedic Journal of China

基　　金：战创伤损伤修复技术与器械研究项目(编号:AWS17J004-02)。

摘　　要：[目的]利用兔桡骨临界骨缺损模型,研究不同剂量的重组人骨发生蛋白2(recombinant human bone morphogenetic protein-2,rhBMP-2)对骨缺损修复的量效关系。[方法]将36只新西兰白兔建立单侧桡骨骨缺损模型,随机分为空白组、海绵空白组、自体骨组、低剂量组(0.25 mg rhBMP-2)、中剂量组(0.5 mg rhBMP-2)、高剂量组(2.5 mg rhBMP-2),每组6只。对骨缺损给予相应处理。分别于术后4、8周,行X线检查、MicroCT检查和组织学观察评估。[结果]与术后第4周相比,8周时各组Lane Sandhu X线评分均增加,其中,空白组和海绵空白组两个时间点间差异无统计学意义(P>0.05),而自体骨组、低、中、高剂量组的两时间点间差异有统计学意义(P<0.05)。相应时间点,Lane Sandhu X线评分由低至高均为空白组<海绵空白组<低剂量组<自体骨组<中剂量组<高剂量组,差异有统计学意义(P<0.05)。与术后4周相比,8周时6组MicroCT检测的骨矿密度(bone mineral density,BMD)和骨小梁数目(trabecular number,Tb.N)均增加,其中,低、中、高剂量组的两时间点间差异有统计学意义(P<0.05)。相应时间点,BMD和Tb.N均由低至高均为:低剂量组<中剂量组<高剂量组,差异有统计学意义(P<0.05)。组织学观察表明,骨缺损新生骨的成熟度呈rhBMP-2剂量依赖性变化。[结论]在0.25~2.5 mg的剂量范围内,rhBMP-2对兔桡骨临界骨缺损的骨修复效果呈剂量依赖性。[Objective]To explore the dose-effect relationship of recombinant human morphogenetic protein-2(rhBMP-2)in the criti-cal radius bone defect model in rabbit.[Methods]A total of 36 New Zealand white rabbits were randomly divided into blank group,sponge blank group,bone autograft group,low-dose group(0.25 mg rhBMP-2),medium-dose group(0.5 mg rhBMP-2)and high-dose group(2.5 mg rhBMP-2),with 6 rabbits in each group.After a 15 mm radius bone defect was made in rabbit,the corresponding bone defect treatments were conducted respectively.The X-ray check,Micro CT examination and histological observation were performed at 4 and 8 weeks postop-eratively.[Results]Compared with those 4 weeks after surgery,Lane Sandhu X-ray scores increased in all groups at 8 weeks,which were not significantly different between the two time points in the blank group and sponge blank group(P<0.05),while statistically significantly different in the bone autograft group,low,medium and high dose groups(P<0.05).At the corresponding time points,Lane Sandhu X-ray scores ranked from low to high all as:blank group<sponge blank group<low dose group<bone autograft group<medium dose group<high dose group,the difference was statistically significant(P<0.05).Regarding Micro CT assay,the bone mineral density(BMD)and trabecular number(Tb.N)increased in all the 6 groups at 8 weeks compared with those at 4 weeks after surgery,which proved statistically significant between the two time points in the low-dose,medium-dose and high-dose groups(P<0.05).At the coresponding time points,BMD and Tb.N ranked from low to high as:low dose group<medium dose group<high dose group,which was statistically significant(P<0.05).In addition,the histological observation showed that the maturity of new bone was dose-dependent with rhBMP-2.[Conclusion]In the dose range of 0.25 mg to 2.5 mg,the bone repair outcome of rhBMP-2 in the critical radial bone defect is dose-dependent.

关 键 词：兔 临界骨缺损 人重组骨形态发生蛋白-2 明胶海绵 骨修复 

分 类 号：R683.41[医药卫生—骨科学]