旱柳浸提物对金线莲组培苗生根的影响  

Effect of Salix mandshurica Extract on Rooting of Anoectochilus roxburghii Tissue Culture Seedlings

作　　者：但英 陈若 何德 李翠新 DAN Ying;CHEN Ruo;HE De(College of Life Sciences,Southwest Forestry University/Key Laboratory of Forest Biotechnology in Yunnan,Kunming,Yunnan 650224)

机构地区：[1]西南林业大学生命科学学院/云南省高校林木生物技术重点实验室,云南昆明650224

出　　处：《安徽农业科学》2023年第11期25-27,31,共4页Journal of Anhui Agricultural Sciences

基　　金：云南省大学生创新创业训练计划(20180451041);云南省高校林木生物技术重点实验室资助项目(2018KF19);云南省高校林下生物资源保护和利用科技创新团队(51400605)。

摘　　要：用4种不同浓度(0.020、0.025、0.050、0.100 g/L)旱柳浸提物对金线莲组培苗生根情况进行研究,以期改善金线莲组培苗不易生根的现状。结果表明,旱柳浸提物对金线莲生根呈低浓度(0.020、0.025 g/L)促进、高浓度(0.100 g/L)抑制的趋势;相比于对照组,旱柳浸提物培养组的生根率、根长和根量均显著提高,并易对金线莲培养基表面下的茎节上分化出根。The rooting of tissue cultured seedlings of Anoectochilus roxburghii was studied by using four different concentrations(0.020,0.025,0.050 and 0.100 g/L)of Salix mandshurica extracts in order to improve the current situation of difficult rooting of A.roxburghii tissue culture seedlings.The results showed that the extract of S.mandshurica promoted the rooting of A.roxburghii with low concentration(0.020,0.025 g/L)and inhibited with high concentration(0.100 g/L)and could significantly shorten the culture time of tissue culture rooting.Compared with the control group,the rooting rate,root length and root quantity of S.mandshurica extract culture group were significantly improved.And it was easy to differentiate roots on the stem nodes under the surface of A.roxburghii culture medium.

关 键 词：金线莲 旱柳浸提物 组织培养 生根 

分 类 号：S682.31[农业科学—观赏园艺]