菊花珍品梨香菊离体快繁技术研究  

Study on the Technique of in vitro Rapid Propagation of Chrysanthemum curiosa Lixiangju

作　　者：邵会会 SHAO Hui-hui(Garden Institute of Tangshan,Tangshan,Hebei 063000)

机构地区：[1]唐山园林科学研究所,河北唐山063000

出　　处：《安徽农业科学》2023年第11期70-72,103,共4页Journal of Anhui Agricultural Sciences

基　　金：河北省建设科技研究计划项目。

摘　　要：以梨香菊的幼嫩叶片为外植体,MS为基本培养基,研究了不同植物生长调节剂及基本培养基对其愈伤组织诱导、增殖、分化及丛生芽增殖和生根的影响,旨在建立离体快繁体系。结果表明:用MS+6-BA 2.0 mg/L+NAA 0.4 mg/L作为培养基可以较快地建立间接再生体系,诱导愈伤组织;愈伤组织增殖的最佳培养基为MS+6-BA 0.5 mg/L+NAA 1.0 mg/L;MS+6-BA 2.0 mg/L+NAA 0.05 mg/L为最佳的分化培养基,分化的芽数较多,芽体健壮饱满;MS+6-BA 1.0 mg/L最适合丛生芽增殖,增殖系数高,芽体粗壮、质量好;1/2MS+NAA 1.0 mg/L培养基中梨香菊生根最好。The leaves of Lixiangju were used as explant,plant growth regulators and basic mediums were studied on callus induction,multiplication,differentiation and shoot multiplication,rooting and growth.The results showed that use MS+6-BA 2.0 mg/L+NAA 0.4 mg/L as culture medium to establish indirect regeneration system and induce callus more faster and better.MS+6-BA 0.5 mg/L+NAA 1.0 mg/L was the best culture medium for callus multiplication.MS+6-BA 2.0 mg/L+NAA 0.05 mg/L was the best culture medium for callus differentiation,the number of buds was more than some other treats,buds stronger and fuller.The vigor of buds was best.When used MS+6-BA 1.0 mg/L as culture medium for shoot multiplication and growth,multiplication coefficient higher,the length of buds longer significantly than other treats.The best rooting medium was 1/2MS+NAA 1.0 mg/L.

关 键 词：梨香菊 组培 增殖 分化 

分 类 号：S682.1[农业科学—观赏园艺]