基于灰色关联度法评价半枝莲药材等级规格内涵  

Evaluation of the Connotation of Grades and Specifications of Scutellaria barbata Based on the Grey Relational Degree Method

作　　者：路丽 张洪坤 黄玉瑶 郭长达 高贯彪 郝建新 LU Li;ZHANG Hong-kun;HUANG Yu-yao(Bozhou Huqiao Pharmaceutical Co.,Ltd.,Bozhou,Anhui 236800;Sichuan Haoyun Pharmaceutical Co.,Ltd.,Guangyuan,Sichuan 628017)

机构地区：[1]亳州市沪谯药业有限公司,安徽亳州236800 [2]四川豪运药业股份有限公司,四川广元628017 [3]广州市和一医疗科技有限公司,广东广州510970

出　　处：《安徽农业科学》2023年第11期132-137,143,共7页Journal of Anhui Agricultural Sciences

基　　金：安徽省科技重大专项(18030801119);国家中医药管理局中药标准化项目(ZYBZH-Y-AH-03)。

摘　　要：[目的]研究不同产地不同等级半枝莲药材中有效成分的质量情况,首次结合灰色关联度法综合评价半枝莲药材等级规格内涵。[方法]采用高效液相色谱法测定不同产地不同等级半枝莲药材样品中野黄芩苷含量,紫外-可见分光光度法测定总黄酮含量,同时以水溶性浸出物、醇溶性浸出物、叶占比作为评价指标,采用相关性分析等级与有效成分的关联性,并结合灰色关联度法综合评价半枝莲药材等级规格内涵。[结果]叶占比、水溶性和醇溶性浸出物、野黄芩苷、总黄酮与等级均呈不同程度的正相关(P<0.05),而总灰分与等级呈显著负相关(P<0.01),进而说明等级越高质量越好。一等半枝莲的相对关联度除了安徽A-1的为0.366,其余为0.417~0.710,统货的相对关联度除了基地A-2和湖南A-2的相对关联度在0.400以上,其余为0.300~0.386,总体显示一等半枝莲的排名基本靠前。同产地的一等半枝莲的相对关联度均高于统货,整体显示一等半枝莲药材质量优于统货,表明等级越高的内在质量越好。[结论]首次建立了评价半枝莲药材等级规格质量的模型,诠释了半枝莲等级越高内在质量越好的内涵,为半枝莲药材等级内涵的评价提供一种新的评价方法。[Objective]To study the quality of effective components in Scutellaria barbata from different producing areas and different grades,and to comprehensively evaluate the connotation of grade specification of Scutellaria barbata in combination with the grey correlation method for the first time.[Method]The scutellarin content in Scutellaria barbata samples of different grades from different producing areas was determined by high performance liquid chromatography,and the content of total flavonoids was determined by UV-vis spectrophotometry.At the same time,the content of water-soluble extract,alcohol soluble extract and leaf proportion were used as evaluation indicators,the correlation between grades and active ingredients was analyzed by correlation,and the connotation of grades and specifications of Scutellaria barbata was comprehensively evaluated in combination with the grey correlation method.[Result]Leaf proportion,water-soluble and alcohol-soluble extracts,scutellarin and total flavonoids were positively correlated with grade(P<0.05),while total ash was significantly negatively correlated with grade(P<0.01),further indicating that the higher the level,the better the quality.The relative correlation degree of first-class Scutellaria barbata was 0.417-0.710 except for Anhui A-1,which was 0.366.The relative correlation degree of unified goods was 0.300-0.386 except that the relative correlation degree of base A-2 and Hunan A-2 was above 0.40.The relative correlation degree of the first-class Scutellaria barbata of the same origin was higher than that of the standard products,and the overall quality of the first-class Scutellaria barbata was better than that of the standard products,indicating that the higher the level,the better the internal quality.[Conclusion]For the first time,a model for evaluating the grade specification quality of Scutellaria barbata was established,which explained the connotation that the higher the grade of Scutellaria barbata was,the better the quality was,and provided a new method for t

关 键 词：半枝莲 灰色关联度法 等级规格 相对关联度 野黄芩苷 总黄酮 叶占比 

分 类 号：R282.5[医药卫生—中药学]