西藏4种杜鹃属植物内生真菌群落组成及多样性分析  

Analysis on the Community Composition and Diversity of Endophytic Fungi in Four Species of Rhododendron in Tibet

作　　者：王素娟 常子惠 张永娟 卫波宁 德吉[1] WANG Su-juan;CHANG Zi-hui;ZHANG Yong-juan(School of Science,Tibet University,Lhasa,Tibet 850000;Agriculture Research Institute,Tibet Academy of Agriculture and Animal Husbandry Sciences,Lhasa,Tibet 850000)

机构地区：[1]西藏大学理学院,西藏拉萨850000 [2]西藏自治区农牧科学院农业研究所,西藏拉萨850000

出　　处：《安徽农业科学》2023年第11期138-143,共6页Journal of Anhui Agricultural Sciences

基　　金：西藏自治区科技厅中央支持地方项目(XZ2020001YD0003C);2022年中央支持地方高校专项(00060949)。

摘　　要：[目的]挖掘西藏杜鹃花科杜鹃属植物内生真菌资源,研究米林杜鹃(Rhododendron mainlingense)、髯花杜鹃(R.anthopogon)、雪层杜鹃(R.nivale)和雪山杜鹃(R.aganniphum)内生真菌的群落组成及多样性。[方法]采用组织分离法对上述4种杜鹃的根、茎、叶和花内生真菌进行分离,用ITS分子标记方法进行菌株鉴定。[结果]西藏4种杜鹃属植物720个组织块中分离得到336株内生真菌,这些真菌被鉴定为2门5纲10目16科21属55种。4种杜鹃属植物内生真菌多样性指数无显著差异。雪山杜鹃(H′=1.96,D=0.80)和雪层杜鹃(H′=1.81,D=0.77)内生真菌Shannon多样性指数(H′)和辛普森指数(D)高于髯花杜鹃(H′=1.36,D=0.71)和米林杜鹃(H′=1.17,D=0.65)内生真菌多样性。根、茎、叶、花不同部位内生真菌多样性也无显著差异。不同部位的H′和D指数分别为根部H′=2.63、D=0.86,茎H′=2.35、D=0.83,叶H′=2.26、D=0.80,花H′=2.23、D=0.83。[结论]研究结果为挖掘杜鹃中内生真菌资源和药效物质筛选研究提供了基础数据。[Objective]To explore the endophytic fungal resources of Rhododendron genus in the Rhododendron family in Tibet,and to study the community composition and diversity of endophytic fungi in Rhododendron mainlingense,R.anthopogon,R.nivale and R.aganniphum.[Method]The endophytic fungi from roots,stems,leaves and flowers of four species were isolated using tissue isolation method and identified by ITS sequencing.[Result]A total of 336 endophytic fungi were isolated from 720 tissues blocks of 4 species of Rhododendron plants in Tibet.These fungi were identified as 55 species belonging to 2 phyla,5 classes,10 orders,16 families,21 genera and 55 species.There was no significant difference in the diversity index of endophytic fungi among the four Rhododendron species.The diversity shannon index(H′)and simpson index(D)of endophytic fungi in R.aganniphum(H′=1.96,D=0.80)and R.nivale(H′=1.81,D=0.77)were higher than in R.anthopogon(H′=1.36,D=0.71)and R.mainlingense(H′=1.17,D=0.65).There was no significant difference in the diversity of endophytic fungi in different parts of roots,stems,leaves and flowers.The H′index and D index in different tissues as follows:H′=2.63,D=0.86 in roots,H′=2.35,D=0.83 in stems,H′=2.26,D=0.80 in leaves and H′=2.23,D=0.83 in flowers.[Conclusion]The research results provide basic data for the exploration of endophytic fungal resources and screening of pharmacological substances in Rhododendron.

关 键 词：杜鹃 内生真菌 群落组成 多样性 

分 类 号：Q948.1[生物学—植物学]