七氟烷后处理改善脑缺血大鼠的远期学习记忆  

Postconditioning with sevoflurane improves long-term learning and memory after focal cerebral ischemia and reperfusion injury in rats

作　　者：王俊宽[1] 许柯炎 秦瑞鸿 陈高 Wang Junkuan;Xu Keyan;Qin Ruihong;Chen Gao(Department of Neurosurgery,the First Affliated Hospital,Zhengzhou University,Zhengzhou 450052,China;Department of Neurosurgery,2nd Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)

机构地区：[1]郑州大学第一附属医院神经外科,郑州450052 [2]浙江大学医学院附属第二医院神经外科,杭州310009

出　　处：《中华实验外科杂志》2023年第5期877-879,共3页Chinese Journal of Experimental Surgery

基　　金：河南省自然科学基金(162300410307);河南省卫生健康科技英才海外研修工程(HWYX2019052)。

摘　　要：目的利用大鼠大脑中动脉闭塞模型(MCAO),观察七氟烷后处理对脑缺血大鼠远期学习记忆能力的影响。方法雄性SD大鼠随机分为假手术组、对照组和七氟烷组,每组28只,七氟烷组在再灌注开始时吸入1.0最小肺泡浓度(MAC)七氟烷进行后处理。再灌注后28 d,分别用氯化三苯基四氮唑(TTC)染色评估大鼠脑梗死容积,用Morris水迷宫评估大鼠的空间学习记忆能力。组间比较采用单因素或双因素方差分析。结果再灌注28 d七氟烷组的脑梗死容积为37.59%,明显小于对照组的46.21%(F=200.5,P<0.05)。水迷宫实验第5天七氟烷组的逃避潜伏期为16.25 s,明显短于对照组的27.27 s(F=24.03,P<0.05)。结论七氟烷后处理可能通过减小脑梗死容积,改善大鼠远期的空间学习和记忆能力。Objective To investigate the effect of postconditioning with sevoflurane on long-term learning and memory in Sprague-Dawley(SD)rats suffered transient middle cerebral artery occlusion(MCAO).Methods Male SD rats were randomly allocated into 3 groups subjected to MCAO and then treated with sevoflurane at the beginning of reperfusion.The infarct volumes were evaluated on the 28th day by 2,3,5-triphenyltertrazolium chloride(TTC)staining.Spatial learning and memory were examined by Morris water maze.Comparisons were made by one-way ANOVA or two-way ANOVA.Results The infarct volume of sevoflurane group is less than that of control group(37.59%vs.46.21%,F=200.5,P<0.05).In Morris water maze by day 5,rats in sevoflurane groups needed shorter time to find the plat-form than those in the control group(16.25 s vs.27.27 s,F=24.03,P<0.05).Conclusion Our data suggest postconditioning with sevoflurane not only reduced infarct volume but also improved spatial learning and memory for long time.

关 键 词：大脑中动脉闭塞模型 七氟烷 后处理 缺血 

分 类 号：R614[医药卫生—麻醉学]