Tunable catalytic activity of FeWO_(4) nanomaterials for sensitive assays of pyrophosphate ion and alkaline phosphatase activity  

作　　者：Xianqing Tang Jinghuang Chen Mengqian Zhang Jian Sun Xiurong Yang 

机构地区：[1]State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [2]School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China [3]Department of Chemistry,University of Science and Technology of China,Hefei 230026,China

出　　处：《Science China Chemistry》2023年第6期1860-1868,共9页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21974132,22034006,21721003);the Open Project of State Key Laboratory of Supramolecular Structure and Materials(sklssm2023021)。

摘　　要：Alkaline phosphatase(ALP)activity and pyrophosphate ion(PPi)levels are remarkable for the human body functions such as signal transduction pathways and metabolism.Current quantitative methods mainly focus on developing complicated organic substrates or employing unstable metal ions as signal-regulated medium.Herein,we have developed a facile hydrothermal method for preparing Fe WO_(4)nanomaterials with intrinsic peroxidase-like activity and further confirmed that such a catalytic activity could be significantly enhanced by adjusting the size and oxygen vacancy content.More encouragingly,PPi can easily inhibit the catalytic activity of Fe WO_(4),whereas orthophosphate ions(Pi)cannot.Therefore,we constructed an Fe WO_(4)-based colorimetric assay for sensing PPi by means of the classical 3,3′,5,5′-tetramethylbenzidine-peroxidase chromogenic reaction.A facile and reliable ALP activity assay was also designed and developed because of the logical regulation of the peroxidase-like activity of Fe WO_(4)through the ALP-catalyzed hydrolysis of PPi into Pi.Based on the clear mechanism and mimetic-enzyme Fe WO_(4)-catalyzed amplification,the sensing system exhibited excellent performance and was able to evaluate ALP activity in real serum samples and screen for potential ALP inhibitors.The proposed mimetic enzyme-involved colorimetric assay provides an alternative pathway,and Fe WO_(4)nanomaterials with excellent performance have great potential for further biosensing and biomedical applications.

关 键 词：FewO4 nanomaterials mimetic enzyme oxygen vacancy COLORIMETRY pyrophosphate ion alkaline phosphatase 

分 类 号：TQ426.97[化学工程] TB383.1[一般工业技术—材料科学与工程]