IL-4/STAT6通路相关基因对Graves病患者外周血B淋巴细胞水平的影响  

Effects of IL-4/STAT6 pathway related genes on peripheral blood B limphocytes in patients with Graves′disease

作　　者：杨倩 查兵兵 YANG Qian;ZHA Bingbing(Department of Endocrinology,Affiliated Shanghai Municipal Fifth People′s Hospital,Fudan University,Shanghai 200240,China)

机构地区：[1]复旦大学附属上海市第五人民医院内分泌科,上海200240

出　　处：《检验医学与临床》2023年第12期1736-1739,1744,共5页Laboratory Medicine and Clinic

基　　金：上海市闵行区科委自然科学基金项目(2020MHZ077)。

摘　　要：目的探讨白细胞介素-4(IL-4)/信转导和转录激活因子6(STAT6)通路相关基因对Graves病患者外周血B淋巴细胞水平的影响。方法选择2021年1-6月在上海市第五人民医院门诊及病房初发、未使用过抗甲状腺药物治疗的Graves病患者20例作为Graves病组,选择同期在上海市第五人民医院体检健康且性别、年龄匹配的20例健康者作为健康对照组。留取外周血并收集血清,检测甲状腺功能[游离三碘甲状腺原氨酸(FT3)、血清游离甲状腺素(FT4)]、甲状腺抗体[甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)、促甲状腺激素受体抗体(TRAb)]、IL-4等指标。磁珠分选外周血B淋巴细胞,RT-PCR检测IL-4R mRNA的表达水平,流式细胞术检测外周血CD19^(+)、CD19^(+)IL-4R^(+)、CD19^(+)pSTAT6^(+)B淋巴细胞水平。结果与健康对照组相比,Graves病组FT3、FT4、TPOAb、TGAb水平升高(P<0.05),CD19^(+)B淋巴细胞比例明显增加(P<0.05)。与健康者相比,RT-PCR检测显示Graves病患者B淋巴细胞IL-4R mRNA表达增加(P<0.05)。Graves病组血清IL-4水平及外周血CD19^(+)IL-4R^(+)、CD19^(+)pSTAT6^(+)B淋巴细胞水平与健康对照组比较,差异均无统计学意义(P>0.05)。IL-4、CD19^(+)IL-4R^(+)B淋巴细胞、CD19^(+)pSTAT6^(+)B淋巴细胞与FT3、FT4及TPOAb、TGAb、TRAb均无相关性(P>0.05)。结论虽然Graves病患者外周血B淋巴细胞显著增加,但是IL-4/STAT6通路相关基因可能不影响Graves病患者外周血B淋巴细胞增生,与疾病状态也无明显相关性。Objective To investigate the effect of interleukin-4(IL-4)/signal transduction and activator of transcription 6(STAT6)pathway related genes on the level of peripheral blood B lymphocytes in the patients with Graves′disease.Methods A total of 20 patients with Graves′disease initial onset without using antithyroid drugs in the outpatient department and ward of Shanghai Municipal Fifth People′s Hospital from January to June 2021 were selected as the Graves′disease group and contemporaneous 20 sex and age matched healthy subjects undergoing healthy physical examination were selected as the health control group.Peripheral blood was taken and serum was collected to detect the thyroid function indicators[free triiodothyronine(FT3),serum free thyroxine(FT4)],thyroid antibodies[thyroid peroxidase antibody(TPOAb),thyroid globulin antibody(TGAb),thyroid stimulating hormone receptor antibody(TRAb)]and IL-4.The magnetic beads were used to isolate peripheral blood B lymphocytes,and RT-PCR was used to detect the expression level of IL-4R mRNA.The flow cytometry was used to detect the levels of CD19^(+),CD19^(+)IL-4R^(+)and CD19^(+)pSTAT6^(+)B lymphocytes in peripheral blood.Results Compared with the healthy control group,the levels of FT3,FT4,TPOAb and TGAb in the Graves′disease group were increased(P<0.05),and the proportion of CD19^(+)B lymphocytes was significantly increased(P<0.05).Compared with healthy subjects,the expression of IL-4R mRNA in B lymphocytes of patients with Graves′disease was increased by RT-PCR(P<0.05).The serum IL-4 level and peripheral blood CD19^(+)IL-4R^(+)and CD19^(+)pSTAT6^(+)B lymphocytes levels had no statistical difference between the Graves′disease group and the healthy control group(P>0.05).IL-4,CD19^(+)IL-4R^(+)lymphocytes and CD19^(+)pSTAT6^(+)B lymphocytes were not correlated with FT3,FT4 and TPOAb,TGAb,TRAb(P>0.05).Conclusion Although peripheral blood B lymphocytes significantly increases in the patients with Graves′disease,but the IL-4/STAT6 pathway related genes may not aff

关 键 词：GRAVES病 白细胞介素-4 信转导和转录激活因子6 B淋巴细胞 

分 类 号：R446.11[医药卫生—诊断学]