关节腔内注射滇白珠醇提取液治疗急性痛风性关节炎的实验研究  

Experimental study on intra-articular cavity injection of gaultheria yunnanensis alcohol extract in treating acute gouty arthritis

作　　者：姚文琴[1] 覃云璐 党荣敏[1] 王恒[1] 邓莹[1] 王定瑜[1] YAO Wenqin;QIN Yunlu;DANG Rongmin;WANG Heng;DENG Ying;WANG Dingyu(Qiannan Medical College for Nationalities,Duyun,Guizhou 558003,China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558003

出　　处：《检验医学与临床》2023年第12期1755-1758,共4页Laboratory Medicine and Clinic

基　　金：黔南民族医专科研基金(QNYZ 201906)。

摘　　要：目的观察关节腔内注射滇白珠醇提取液对急性痛风性关节炎的治疗作用及可能的机制。方法SD雄性大鼠50只按随机数字表法分为空白对照组、模型组及滇白珠醇提取液高、中、低剂量组。除空白对照组外,其余组大鼠均通过关节腔内注射单钠尿酸盐晶体混悬液制备急性痛风性关节炎模型,造模成功后,滇白珠醇提取液不同剂量组给予不同剂量(4、2、1 g/kg)滇白珠醇提取液0.2 mL关节腔内注射治疗,模型组以同样方法注射等量无菌生理盐水,持续1周后,各组大鼠取外周血采用酶联免疫吸附试验检测血清白细胞介素(IL)-17、血管内皮生长因子(VEGF)、IL-6水平,同时取踝关节组织进行病理学检查。结果与模型组相比,滇白珠醇提取液不同剂量组均可明显降低血清中IL-17、VEGF、IL-6水平,差异均有统计学意义(P<0.05)。滇白珠醇提取液中、高剂量组血清IL-17、VEGF、IL-6水平均低于滇白珠醇提取液低剂量组,差异均有统计学意义(P<0.05);滇白珠醇提取液高剂量组血清IL-17、VEGF水平与滇白珠醇提取液中剂量组相比,差异均无统计学意义(P>0.05);滇白珠醇提取液高剂量组血清IL-6水平低于滇白珠醇提取液中剂量组,差异有统计学意义(P<0.05)。踝关节病理结果显示:治疗后,滇白珠醇提取液低剂量组纤维组织及滑膜轻度增生;滇白珠醇提取液中剂量组较多淋巴细胞、浆细胞浸润,色素沉着,滑膜轻度增生;滇白珠醇提取液高剂量组肉芽组织增生,大量淋巴细胞、浆细胞浸润,滑膜未见增生,关节软骨及骨组织结构形态正常,未见痛风结节。结论关节腔内注射滇白珠醇提取液对急性痛风性关节炎有治疗效果,且呈现剂量依赖性,可能与下调血清IL-17、VGEF、IL-6等炎症因子水平有关。Objective To observe the therapeutic effect and possible mechanism of intra-articular injection of gaultheria yunnanensis alcohol extract on acute gouty arthritis.Methods Fifty SD male rats were randomly divided into the blank control group,model group,and high,medium and low dose groups of gaultheria yunnanensis alcohol extract.Except for the blank control group,the rats in the other groups were used to prepare the acute gouty arthritis models by intra-articular injection of monosodium urate crystal suspension.After successful modeling,different dosage groups of gaultheria yunnanensis alcohol extract were treated by intra-articular injection of different doses(4,2,1 g/kg)of 0.2 mL gaultheria yunnanensis alcohol extract.The model group was injected with the same amount of sterile physiological saline using the same method.After one week of continuous use,the peripheral blood in each group was collected for detecting serum interleukin(IL)-17,vascular endothelial growth factor(VEGF)and IL-6 levels by enzyme-linked immunosorbent assay.At the same time,the ankle joint tissue was taken for conducting the pathological examination.Results Compared with the model group,different doses groups of gaultheria yunnanensis alcohol extract could significantly reduce the serum IL-17,VEGF and IL-6 levels,and the differences were statistically significant(P<0.05).The serum IL-17,VEGF and IL-6 levels in the yunnanensis alcohol extract middle and high dose groups were lower than those in the yunnanensis alcohol extract low dose group,and the differences were statistically significant(P<0.05);there was no statistically significant difference in serum IL-17 and VEGF levels between the yunnanensis alcohol extract high dose group and middle dose group(P>0.05);the serum IL-6 level in the yunnanensis alcohol extract high-dose group was lower than that in the yunnanensis alcohol extract middle-dose group,and the difference was statistically significant(P<0.05).The pathological results ankle joint showed that after treatment,the yunnanensis

关 键 词：滇白珠 急性痛风性关节炎 炎症因子 

分 类 号：R285.5[医药卫生—中药学]