Herbal small RNAs in patients with COVID-19 linked to reduced DEG expression  

作　　者：Xiangyu Qiao Fengming Huang Xiaohu Shi Xingyu Deng Cong Zhang Song Mei Zhiqing Wang Congzhao Zhou Chengyu Jiang Xinghua Tan 

机构地区：[1]State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,Department of Biochemistry,School of Basic Medicine Peking Union Medical College,Beijing,100005,China [2]Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing,100730,China [3]Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences,University of Science and Technology of China,Hefei,230027,China [4]Guangzhou Eighth People’s Hospital,Guangzhou Medical University,Guangzhou,510440,China

出　　处：《Science China(Life Sciences)》2023年第6期1280-1289,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (81788101,32100104);the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (2021-I2M-1-022);the CAMS Endowment Fund (2021-CAMS-JZ001);the Overseas Expertise Introduction Center for Discipline Innovation (“111 Center”) (BP0820029);the National Science and Technology Major Project (2018ZX10102-001-005-002)。

摘　　要：In China,more than 80%of patients with coronavirus disease 2019(COVID-19)received traditional Chinese medicine(TCM)as a treatment and their clinical efficacy have been reported.However,the underlying molecular mechanism remains unclear.Previous studies have identified herbal small RNAs(s RNAs)as novel functional components.In this study,a cohort of 22patients with COVID-19 treated with Toujie Quwen(TQ)granules was analyzed.We observed thousands of herbal small RNAs that entered the blood cells of patients after the consumption of TQ granules.In response to this treatment,the reduced differentially expressed genes(DEGs)were highly correlated with the predicted target genes of the most prevalent herbal s RNAs detected in the blood.Moreover,the predicted target genes of the top 30 s RNAs from each of the 245 TCMs in the Bencao s RNA Atlas overlapped with 337 upregulated DEGs in patients with mild COVID-19,and 33 TCMs,with more than 50%overlapping genes were identified as effective TCMs.These predicted target genes of top 30 s RNAs from Juhong,Gualoupi and Foshou were confirmed experimentally.Our results not only elucidated a novel molecular mechanism of TCM potential clinical efficacy for COVID-19 patients,but also provided 33 effective COVID-19 TCMs for prescription optimization.

关 键 词：traditional Chinese medicine Toujie Quwen granules CovID-19 TRANSCRIPTOME 

分 类 号：R259[医药卫生—中西医结合]