柯萨奇病毒A组10型感染RD细胞的转录组学分析  

Transcriptomics analysis of RD cells infected with coxsackievirus A10

作　　者：江俊涛 任盈盈 刘洪波 JIANG Juntao;REN Yingying;LIU Hongbo(Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders,The Second Affiliated Hospital of Guilin Medical University,Guilin 541199,Guangxi,China;College of Clinical Laboratory Science of Guilin Medical University,Guilin 541199,Guangxi,China;State Key Laboratory of Virology,College of life sciences at Wuhan University,Wuhan 430072,Hubei,China)

机构地区：[1]桂林医学院第二附属医院广西糖脂代谢病重点实验室,广西桂林541199 [2]桂林医学院医学检验学院,广西桂林541199 [3]武汉大学生命科学学院,病毒学国家重点实验室,湖北武汉430072

出　　处：《生物资源》2023年第2期185-192,共8页Biotic Resources

基　　金：国家自然科学基金(81660280)。

摘　　要：探讨柯萨奇病毒A组10型(coxsackievirus A10,CV⁃A10)感染人横纹肌肉瘤(rhabdomyosarcoma,RD)细胞的基因表达谱变化,为深入理解CV⁃A10致病机制提供重要基础数据。本研究将CV⁃A10以感染复数(MOI)为0.1的剂量感染RD细胞12 h后提取总RNA,通过RNA⁃Seq技术获取RD细胞中的全部转录本信息,基于生物信息学的方法对差异表达基因进行GO(gene ontology,GO)和KEGG(Kyoto encyclopedia of genes and genomes,KEGG)分析。结果显示,共筛选到2610个差异表达基因,其中表达上调的基因有1582个,表达下调的基因有1028个。随机选取8个差异表达基因,经RT⁃qPCR验证后发现,其变化趋势与转录组数据一致。GO和KEGG富集分析结果显示,差异表达基因主要聚焦于细胞内信号转导、mRNA加工、抗病毒免疫反应等过程。结果表明,CV⁃A10感染可能会引起RD细胞发生自噬。To explore the changes of gene expression profile in human rhabdomyosarcoma(RD)cells infected with coxsackievirus A10(CV⁃A10),and to provide important basic data for further understanding the pathogenesis of CV⁃A10,RD cells were infected with CV⁃A10 at a multiplicity of infection(MOI)of 0.1 for 12 h,and total RNA was ex⁃tracted.All transcriptional information of infected RD cells was obtained though RNA⁃Seq technology.Based on bioinfor⁃matics methods,differentially expressed genes were analyzed using GO(gene ontology)and KEGG(Kyoto encyclopedia of genes and genomes).The results showed that a total of 2610 differentially expressed genes were screened,of which 1582 were up⁃regulated and 1028 were down⁃regulated.8 differentially expressed genes were randomly selected and veri⁃fied by RT⁃qPCR,and the variation trend was consistent with the transcriptome data.The enrichment analysis results of GO and KEGG showed that the differentially expressed genes mainly focused on intracellular signal transduction,mRNA processing,antiviral immune response,and other processes.This study suggests that CV⁃A10 infection may cause au⁃tophagy in RD cells.

关 键 词：柯萨奇病毒A组10型 人横纹肌肉瘤细胞 转录组学 

分 类 号：R373.2[医药卫生—病原生物学]