ERα在雌激素调控颏舌肌功能中的作用研究  

The role of ERαin the regulation of genioglossus muscle function by estrogen

作　　者：李环[1] 侯玉霞[1] LI Huan;HOU Yuxia(Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases,College of Stomatology,Xi’an Jiaotong University,710004 China)

机构地区：[1]西安交通大学口腔医院,陕西省颅颌面精准医学研究重点实验室,陕西省牙颌疾病临床研究中心,710004

出　　处：《实用口腔医学杂志》2023年第3期296-299,共4页Journal of Practical Stomatology

摘　　要：目的:探索ERα在雌激素调控SD大鼠颏舌肌功能中的作用。方法:将30只8周龄雌性SD大鼠(250±25)g,随机分为假手术组(SHAM组),去卵巢+雌二醇组(OVX+E2组),去卵巢+雌二醇+ERα拮抗剂MPP组(OVX+E2+MPP组)(n=10)。通过卵巢切除与雌激素回补,在外周血建立稳定的雌激素水平大鼠模型;使用玻璃微电极检测SD大鼠舌下神经核团放电频率,BL-420E+生物机能实验系统检测颏舌肌肌电频率及幅值。结果:(1)舌下神经核放电频率:OVX+E2+MPP组明显弱于OVX+E2组(P<0.05)。(2)颏舌肌电频率及幅值:OVX+E2+MPP组明显弱于OVX+E2组(P<0.05)。结论:雌激素可能通过与ERα受体结合,影响舌下神经核放电,进而改变颏舌肌肌电功能。Objective:To explore the role of ERαin the regulation of genioglossus muscle function by estrogen in SD rats.Methods:308-week-old female SD rats(250±25)g were randomly divided into 3 groups(n=10),including sham operation group(sham group),ovariectomy+estradiol group(OVX+E 2 group),ovariectomy+estradiol+ERαantagonist MPP group(OVX+E 2+MPP group).Through ovariectomy and estrogen replenishment,the rat model with stable estrogen level in the peripheral blood was established.The hypoglossal nucleus discharge frequency of the rats was measured by glass microelectrode,the myoelectric frequency and amplitude of genioglossus muscle were detected by BL-420E+biological function experimental system.Results:(1)The discharge frequency of the hypoglossal nucleus in OVX+E 2+MPP group was significantly lower than that in OVX+E 2 group(P<0.05).(2)The myoelectric frequency and amplitude of genioglossus muscle in OVX+E 2+MPP group were significantly weaker than those in the OVX+E 2 group(P<0.05).Conclusion:Estrogen may affect the discharge of the hypoglossal nucleus through binding with ERαreceptor,and then change the activities of genioglossus in rats.

关 键 词：OSAHS 颏舌肌 舌下神经核 ERα 雌激素受体 

分 类 号：R766[医药卫生—耳鼻咽喉科]