热毒宁直肠给药对幼年大鼠的急性毒性及直肠黏膜刺激实验研究  

Acute Toxicity and Rectal Mucosal Irritation of Reduning on Juvenile Rats by Rectal Administration

作　　者：李妙媛 唐坤泉 李映妮 熊磊 LI Miaoyuan;TANG Kunquan;LI Yingni;XIONG Lei(Liuzhou Maternity and Child Healthcare Hospital/Affiliated Maternity Hospital and Affiliated Children's Hospital of Guangxi University of Science and Technology,Liuzhou 545001,China;Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]柳州市妇幼保健院/广西科技大学附属妇产医院/儿童医院,广西柳州545001 [2]云南中医药大学,云南昆明650500

出　　处：《云南中医药大学学报》2023年第2期90-93,共4页Journal of Yunnan University of Chinese Medicine

基　　金：广西自然科学基金项目(2020GXNSFAA297169);广西中医药适宜技术开发与推广项目(GZSY20-57);云南省科技厅重点研发计划(202103AC100005);柳州市科技项目(2020NBAB0802)。

摘　　要：目的 对热毒宁注射液直肠给药的急性毒性及直肠黏膜刺激性进行实验研究,为临床热毒宁灌肠使用安全提供参考。方法 将40只幼鼠随机分为急性毒性对照组及实验组、直肠黏膜刺激对照组及实验组,急性毒性实验组每次直肠给药20 mL/kg,间隔2 h重复给药共3次,对照组以等量0.9%生理盐水灌肠,观察7 d后幼鼠一般情况、体质量及死亡幼鼠数。直肠黏膜刺激实验组根据临床用药量每天予直肠给药2 mL/kg,每日1次,直肠黏膜刺激对照组以等量0.9%生理盐水灌肠,每日1次。两组连续给药7 d后观察对直肠黏膜刺激情况。结果急性毒性实验组幼鼠灌肠后体质量与对照组比较无统计学意义(P>0.05),灌肠后7 d幼鼠无中毒症状、无死亡。直肠黏膜刺激实验组幼鼠直肠黏膜刺激性与对照组刺激性水平相当,黏膜未见损伤及分泌物,部分可见轻中度充血。结论 热毒宁注射液直肠给药安全无毒性反应,对直肠黏膜有轻度刺激而无损伤。Objective The acute toxicity and rectal mucosal irritation of Reduning Injection enema were studied in order to provide reference for clinical safety of Reduning enema.Methods Forty juvenile rats were randomly divided into acute toxicity control group and experimental group,rectal mucosal stimulation control group and experimental group.The acute toxicity group was given rectal administration of 20 mL/kg each time,repeated three times at an interval of 2 hours,and the control group was given an equal amount of 0.9%normal saline enema.The general condition,weight and number of dead juvenile rats were observed after 7 days.The rectal mucosal stimulation group was given rectal administration of 2 mL/kg per day,according to the clinical dosage,and the rectal mucosal stimulation was observed after continuous administration for 7 days.Results There was no significant difference in body mass between the acute toxicity experimental group and the control group after enema(P>0.05).There were no poisoning symptoms and no death of the juvenile rats 7 days after enema.Rectal mucosa irritation of juvenile rats in the experimental group was similar to that in the control group.No injury or secretion was observed in the mucosa,and some mild to moderate hyperemia was observed.Conclusion The rectal administration of Reduning Injection is safe and non-toxic,with mild irritation to the rectal mucosa without damage.

关 键 词：热毒宁 大鼠 直肠给药 急性毒性 直肠黏膜刺激性 

分 类 号：R285.5[医药卫生—中药学]