金银花表面微生物的分离、鉴定及抑菌活性研究  

Isolation,identification and antibacterial activity of surface microorganisms of Lonicera Japonica Flos

作　　者：周娜 任广喜[1] 姜丹[1] 李婷 申小营 华国栋 刘春生[1] ZHOU Na;REN Guang-xi;JIANG Dan;LI Ting;SHEN Xiao-ying;HUA Guo-dong;LIU Chunsheng(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京中医药大学东直门医院,北京100700

出　　处：《中南药学》2023年第6期1486-1490,共5页Central South Pharmacy

基　　金：北京中医药大学横向课题项目(No.2020110031010303)。

摘　　要：目的研究金银花的表面微生物的组成和抑菌功能,一方面为金银花药材表面可能存在的污染菌提供参考,另一方面对其功能菌进行深入挖掘。方法以河南封丘、山东平邑、河北巨鹿三个地区的金银花药材为研究对象,利用微生物培养技术,分离并鉴定其种属,并在此基础上检测其抑菌活性。结果金银花表面菌群丰富,分离得到表面微生物53株,分布于5门7纲8目12科15属;群落组成显示,存在镰刀菌属、链格孢属、裂褶菌属、青霉属等污染菌;在抑菌拮抗实验中得到一株真菌,鉴定为非洲哈茨木霉,其能够抑制枯草芽孢杆菌。结论金银花表面既存在污染菌,同时也存在功能菌,为防治真菌的污染和反向挖掘微生物的功能提供理论依据。Objective To determine the composition and antibacterial function of surface microorganism in Lonicera Japonica Flos,provide reference for possible contaminating bacteria on the surface of Lonicera Japonica Flos,and explore the functional microorganisms.Methods Lonicera Japonica Flos medicinal materials from Fengqiu in Henan,Pingyi in Shandong,and Julu in Hebei were selected as the research objects.Microbial isolation and identification were conducted with microbiological cultivation technology,and their antibacterial activity was measured.Results It showed that the surface microorganism of Lonicera Japonica Flos was rich.We isolated 53 strains of surface microorganisms,belonging to 5 phyla,7 classes,8 orders,12 families,and 15 genera.The community composition showed the existence of contaminating bacteria such as Fusarium,Alternaria,Cladosporium,and Penicillium.In addition,one fungus was detected in the antibacterial antagonistic experiment,identified as Trichoderma afroharzianum,which inhibited Bacillus subtilis.Conclusion Both contaminating and functional microorganisms exist on the surface of Lonicera Japonica Flos,providing theoretical basis for preventing fungal contamination and reversely explorating microbial functions.

关 键 词：金银花 表面微生物 分离鉴定 抑菌功能 

分 类 号：R379.9[医药卫生—病原生物学]